Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction

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dc.citation.volume10]
dc.contributor.authorAnatriello, Elen [UNIFESP]
dc.contributor.authorFreire Oliveira, Carlo Jose
dc.contributor.authorOliveira, Nathalia Baptista
dc.contributor.authorFisch, Andressa
dc.contributor.authorMilanezi, Cristiane Maria
dc.contributor.authorda Silva, Joao Santana
dc.contributor.authorFerreira de Miranda-Santos, Isabel Kinney
dc.contributor.authorFerreira, Beatriz Rossetti
dc.coverageLondon
dc.date.accessioned2020-06-26T16:30:16Z
dc.date.available2020-06-26T16:30:16Z
dc.date.issued2017
dc.description.abstractBackground: It has recently been demonstrated that saliva from Rhipicephalus sanguineus ticks contains adenosine (ADO) and prostaglandin E2 (PGE2), two non-protein molecules that have significant immunomodulatory properties. These molecules can inhibit cytokine production by dendritic cells (DCs), while also reducing the expression of CD40 in these cells. However, more studies are needed for a better understanding of their participation in the feeding of ticks in vivo. This work, therefore, evaluated the importance of ADO during tick infestations. Mice were infested with adult ticks (3 couples/mouse), and their skin was collected at the tick-infested site (3rd and 7th day), and mRNA for receptors of ADO was quantified by real-time PCR. Results: Tick infestation increased by four and two times the expression of the A2b and A3v1 receptors on day 3, respectively, while expression of other ADO receptors was unaltered. In addition, we treated mice (n = 10/group) daily with 8-(p-Sulfophenyl) theophylline, 8-pSPT, 20 mg/kg, i.p.), a non-selective antagonist of ADO receptors, and evaluated the performance of ticks during infestations. Female ticks fed on 8-pSPT-treated mice presented a reduction in their engorgement, weight and hatching rates of egg masses, and survival times of larvae compared to the same parameters presented by ticks in the control group. To investigate if these 8-pSPT-treated mice presented altered immune responses, we performed three tick infestations and collected their lymph node cells to determine the percentages and activation state of DCs and cytokine production by lymphocytes by flow cytometry (Cytometric Bead Array technique, CBA). Our data showed that 8-pSPT-treated mice presented an increase in the percentage of DCs as well as of their stimulatory and co-stimulatory molecules (CD40, CD80 and MHCII). Regarding production of T cell cytokines, we observed a significant increase in the levels of IL-2 and a significant decrease in IL-10, IL-17, TNF-alpha and IFN-gamma cytokines. Conclusions: These results suggest that ADO produced by ticks helps them feed and reproduce and that this effect may be due to modulation of host DCs and T cells.en
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Inst Sci & Technol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Fed Triangulo Mineiro, UFTM, Inst Biol & Nat Sci, Praca Manoel Terra 330, BR-38015050 Uberaba, MG, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Sch Nursing, Dept Maternal & Child & Publ Hlth Nursing, Ave Bandeirantes 3900, BR-14040902 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Sch Nursing, Dept Biochem & Immunol, Ave Bandeirantes 3900, BR-14040900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Inst Sci & Technol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipIDCNPq: 301,663/2007-6
dc.description.sponsorshipIDCNPq: 308,815/2010-6
dc.description.sponsorshipIDCNPq: 308,280/2013-0
dc.description.sponsorshipIDFAPESP: 2011/00905-8
dc.description.sponsorshipIDFAPESP: 2010/11285-8
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s13071-017-2248-8]
dc.identifier.citationParasites & Vectors. London, v. 10, p. -, 2017.
dc.identifier.doi10.1186/s13071-017-2248-8
dc.identifier.fileWOS000405902200001.pdf
dc.identifier.issn1756-3305
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53456
dc.identifier.wosWOS:000405902200001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofParasites & Vectors
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTicksen
dc.subjectRhipicephalus sanguineusen
dc.subjectSalivaen
dc.subjectAdenosineen
dc.subjectDendritic cellsen
dc.subjectT cellsen
dc.titleInteraction between saliva's adenosine and tick parasitism: effects on feeding and reproductionen
dc.typeinfo:eu-repo/semantics/article
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