The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation

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dc.contributor.authorRisso, Patricia Sayuri
dc.contributor.authorKoike, Marcia Kiyomi
dc.contributor.authorAbrahão, Marcos de Souza [UNIFESP]
dc.contributor.authorFerreira, Natalie Chaves
dc.contributor.authorMontero, Edna Frasson de Souza [UNIFESP]
dc.contributor.institutionUniversity City of São Paulo
dc.contributor.institutionSão Paulo University Medical School
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:53Z
dc.date.available2015-06-14T13:46:53Z
dc.date.issued2014-01-01
dc.description.abstractPURPOSE:To evaluate the NAC effects on liver hypothermic preservation at different time intervals.METHODS:For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal.RESULTS: The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells.CONCLUSION: Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury.en
dc.description.affiliationUniversity City of São Paulo
dc.description.affiliationSão Paulo University Medical School
dc.description.affiliationFederal University of São Paulo Paulista Medicine School
dc.description.affiliationUnifespUNIFESP, Paulista Medicine School
dc.description.sourceSciELO
dc.format.extent28-32
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001700006
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 28-32, 2014.
dc.identifier.doi10.1590/S0102-86502014001700006
dc.identifier.fileS0102-86502014001500028.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001500028
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8193
dc.identifier.wosWOS:000344466300007
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcetylcysteineen
dc.subjectLiveren
dc.subjectHypothermiaen
dc.subjectOrgan Preservationen
dc.subjectRatsen
dc.titleThe effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservationen
dc.typeinfo:eu-repo/semantics/article
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