Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization

dc.contributor.authorCerqueira, Aloysio de Mello Figueiredo
dc.contributor.authorTibana, Anita
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2018-06-15T17:58:46Z
dc.date.available2018-06-15T17:58:46Z
dc.date.issued1994-08-01
dc.description.abstractA total of 1,066 Escherichia call colonies isolated from 105 raw bovine meat samples purchased at supermarkets in Rio de Janeiro were submitted to hybridization assays with gene probes for LT-II and STb enterotoxins. Five colonies comprising four different E. call strains isolated from four pieces of beef, two samples of ground beef (5.7%) and two hamburger patties (5.7%) hybridized with the LT-II probe, while no hybridization occurred with the STb probe. Expression of LT-II enterotoxin using the Y1 adrenal cell assay was verified in two of four E. coli strains. A serotype diversity existed among LT-II E. call strains.en
dc.description.affiliationESCOLA PAULISTA MED, DEPT MICROBIOL IMMUNOL & PARASITOL, BR-04023 SAO PAULO, BRAZIL
dc.description.affiliationUNIV FED RIO DE JANEIRO, INST MICROBIOL, DEPT MED MICROBIOL, BR-21941 RIO DE JANEIRO, BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED, DEPT MICROBIOL IMMUNOL & PARASITOL, BR-04023 SAO PAULO, BRAZIL
dc.description.sourceWeb of Science
dc.format.extent734-736
dc.identifierhttps://dx.doi.org/10.4315/0362-028X-57.8.734
dc.identifier.citationJournal Of Food Protection. Des Moines: Int Assoc Food Protection, v. 57, n. 8, p. 734-736, 1994.
dc.identifier.doi10.4315/0362-028X-57.8.734
dc.identifier.issn0362-028X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/44364
dc.identifier.wosWOS:A1994PF22300015
dc.language.isoeng
dc.publisherInt Assoc Food Protection
dc.relation.ispartofJournal Of Food Protection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLT-IIen
dc.subjectSTBen
dc.subjectESCHERICHIA COLIen
dc.subjectBOVINE MEATen
dc.titleSearch for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridizationen
dc.typeinfo:eu-repo/semantics/article
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