Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection

Página do item simplificado
dc.citation.volume68
dc.contributor.authorTavares Mascagni, Daniela Branco
dc.contributor.authorMiyazaki, Celina Massumi
dc.contributor.authorda Cruz, Nilson Cristino
dc.contributor.authorMoraes, Marli Leite de [UNIFESP]
dc.contributor.authorRiul, Antonio, Jr.
dc.contributor.authorFerreira, Marystela
dc.coverageAmsterdam
dc.date.accessioned2020-07-31T12:47:31Z
dc.date.available2020-07-31T12:47:31Z
dc.date.issued2016
dc.description.abstractWe report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS. Stable aqueous dispersions of GPDDA and GPSS are easily obtained, enabling the growth of LbL films on various solid supports. The use of graphene in the immobilization of GOx promoted Direct Electron Transfer, which was evaluated by Cyclic Volt-ammetry. Amperometric measurements indicated a detection limit of 13.4 mu mol.L-1 and sensitivity of 2.47 mu A.cm(-2). mmol(-1).L for glucose with the (GPDDA/GPSS)(1)/(GPDDA/GOx)(2) architecture, whose thickness was 19.80 +/- 0.28 nm, as determined by Surface Plasmon Resonance (SPR). The sensor may be useful for clinical analysis since glucose could be detected even in the presence of typical interfering agents and in real samples of a lactose-free milk and an electrolyte solution to prevent dehydration. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.affiliationSao Paulo State Univ UNESP, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Carlos, UFSCar, Campus Sorocaba, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Unifesp, Campus Sao Jose dos Campos, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Campinas Unicamp, Sao Paulo, Brazil
dc.description.affiliationUnifespInstituto de Ciência e Tecnologia, Universidade Federal de São Paulo UNIFESP, Rua Talim, 330, 12231-280 São José dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2014/17519-1
dc.description.sponsorshipIDFAPESP: 2014/15093-7
dc.format.extent739-745
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2016.06.001
dc.identifier.citationMaterials Science & Engineering C-Materials For Biological Applications. Amsterdam, v. 68, p. 739-745, 2016.
dc.identifier.doi10.1016/j.msec.2016.06.001
dc.identifier.issn0928-4931
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56882
dc.identifier.wosWOS:000382600000087
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applications
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectGlucose oxidase biosensoren
dc.subjectDirect electron transferen
dc.subjectSelf-assembled filmsen
dc.subjectFunctionalized rGOen
dc.titleLayer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detectionen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICT - Artigos