Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse

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dc.citation.issue3
dc.citation.volume231
dc.contributor.authorPreite, Nailliw Zanini [UNIFESP]
dc.contributor.authorNascimento, Bruna Pascarelli Pedrico do [UNIFESP]
dc.contributor.authorMuller, Cynthia R.
dc.contributor.authorAmerico, Anna Laura V.
dc.contributor.authorHiga, Talita S.
dc.contributor.authorEvangelista, Fabiana S.
dc.contributor.authorLancellotti, Carmen L.
dc.contributor.authorHenriques, Felipe dos Santos
dc.contributor.authorBatista, Miguel Luiz, Jr.
dc.contributor.authorBianco, Antonio C.
dc.contributor.authorRibeiro, Miriam O.
dc.coverageBristol
dc.date.accessioned2020-07-31T12:47:14Z
dc.date.available2020-07-31T12:47:14Z
dc.date.issued2016
dc.description.abstractThe brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through beta-adrenergic receptors (AR). Here, we wished to define the role played by the AR beta(3) isoform in this process. This study focused on the AR beta(3) knockout mice (AR beta 3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). AR beta 3KO mice defend core temperature during cold exposure (4 degrees C for 5h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, AR beta 3KO mice kept on a high-fat diet (HFDen
dc.description.abstract40% fat) for 8 weeks exhibited greater susceptibility to diet-induced obesity, markedly increased epididymal adipocyte area with clear signs of inflammation. The HFD-induced glucose intolerance was similar in both groups but serum hypertriglyceridemia and hypercholesterolemia were less intense in AR beta 3KO animals when compared with WT controls. Isoproterenol-induced lipolysis in isolated white adipocytes as assessed by glycerol release was significantly impaired in AR beta 3KO animals despite normal expression of key proteins involved in lipid metabolism. In conclusion, AR beta(3) inactivation does not affect BAT thermogenesis but increases susceptibility to diet-induced obesity by dampening WAT lipolytic response to adrenergic stimulation.en
dc.description.affiliationUniv Prebiteriana Mackenzie, Ctr Biol & Hlth Sci, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Translat Med, EPM, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Expt Pathophysiol Dept, Fac Med, BR-05508 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo, SP, Brazil
dc.description.affiliationSch Med Sci, Dept Pathol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Integrated Grp Biotechnol, Lab Adipose Tissue Biol, Mogi Das Cruzes, SP, Brazil
dc.description.affiliationRush Univ & Med Ctr, Dept Internal Med, Div Endocrinol & Metab, Chicago, IL USA
dc.description.affiliationUnifespDepartment of Translational Medicine, EPM, Universidade Federal de São Paulo (UNIFESP), Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2009/50353-1
dc.description.sponsorshipIDFAPESP: 2015/19259-0
dc.format.extent259-269
dc.identifierhttp://dx.doi.org/10.1530/JOE-16-0199
dc.identifier.citationJournal Of Endocrinology. Bristol, v. 231, n. 3, p. 259-269, 2016.
dc.identifier.doi10.1530/JOE-16-0199
dc.identifier.issn0022-0795
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56682
dc.identifier.wosWOS:000387982200009
dc.language.isoeng
dc.publisherBioscientifica Ltd
dc.relation.ispartofJournal Of Endocrinology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectobesityen
dc.subjectlipolysisen
dc.subjectadaptive thermogenesisen
dc.subjectbrown adipose tissueen
dc.subjectbeta(3) adrenergic receptoren
dc.titleDisruption of beta3 adrenergic receptor increases susceptibility to DIO in mouseen
dc.typeinfo:eu-repo/semantics/article
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