Estudo morfológico do intestino delgado de ratos submetido à oxigenação hiperbárica hipotérmica
Arquivos
Data
2007
Autores
Guimaraes, Fernando Augusto Garcia 
Orientadores
Taha, Murched Omar
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Avaliar o grau de lesao do intestino delgado submetido a preservacao hipotermica e oxigenacao hiperbarica (OHB). Metodos: Foram utilizados 20 ratos Wistar machos, com peso medio de 264,33 g e idade de 3 meses. Apos irrigacao intraluminal e perfusao vascular, segmentos jejunais de 3cm de cada animal foram mantidos em Ringer lactato a temperatura entre 2 e 4°C (RLg) e distribuidos aleatoriamente em 4 grupos: Grupo Ia = acondicionamento em geladeira por 12 horas (n=10); Grupo Ib = aplicacao de OHB refrigerada por 12 horas (n=lO); Grupo lIa = acondicionamento em geladeira por 24 horas (n=lO); Grupo IIb = aplicacao de OHB refrigerada por 24 horas (n=lO). Avaliaram-se o grau de lesao da mucos a e a altura das vilosidades por H.E., bem como os indices de apoptose e de mitose pelo metodo de TUNEL e por expressao do PCNA, respectivamente. Resultados: Nos Grupos Ia e lIa, observaram-se maiores graus de lesao (Graus 6 e 7), com significante diminuicao da espessura da camada mucosa e aumento do indice de apoptose (p<O,OI) quando comparados com os Grupos Ib e IIb, caracterizando a melhor condicao de preservacao com a aplicacao da OHB. Os indices de apoptose no Grupo Ib foram menores do que no Grupo IIb (p<O,OI). Os indices mitoticos foram maiores nos segmentos preservados na camara hiperbarica, se comparados aos acondicionados na geladeira (p<0,05), indicando que a OHB mantem a potencial idade de recuperacao da mucosa. Conclusao: A oxigenacao hiperbarica protege o intestino delgado de rato das lesoes decorrentes da preservacao hipotermica
The aim of this work was to evaluate the range of mucosal injury in small bowel submitted to hypothermic preservation and hyperbaric oxygenation (HBO). Methods: Twenty 3-month-old male Wistar rats weighting 240 to 300 g were used as bowel donors. After luminal flush and vascular perfusion, 3-cm segments were maintained in Ringer’s solution at temperatures between 2°C to 4°C and randomly divided into 4 groups: Group Ia = conditioned in cold storage for 12 hours; Group Ib = conditioned in refrigerating HBO chamber for 12 hours (n=10); Group IIa = conditioned in cold storage for 24 hours (n=10); Group IIb = conditioned in refrigerating HBO chamber for 24 hours (n=10). After these preservation times, we studied intestinal tissue injury and morphometric analysis of the villi by H.E; apoptotic index by TUNEL and mitotic index by PCNA expression. Results: The grade of the lesions was significantly greater among group Ia and Ib compared to groups IIa and IIb, with smaller villi height and greater apoptotic index (p < 0,01). The apoptotic index was significantly smaller in group Ib compared to group IIb (p < o,01). The mitotic index was greater in segments preserved in hyperbaric chamber compared to conditioned in cold storage (p<0,05). Conclusion: The hyperbaric oxygenation protects the rat small bowel of the lesions in cold storage and preserves its structure adequately
The aim of this work was to evaluate the range of mucosal injury in small bowel submitted to hypothermic preservation and hyperbaric oxygenation (HBO). Methods: Twenty 3-month-old male Wistar rats weighting 240 to 300 g were used as bowel donors. After luminal flush and vascular perfusion, 3-cm segments were maintained in Ringer’s solution at temperatures between 2°C to 4°C and randomly divided into 4 groups: Group Ia = conditioned in cold storage for 12 hours; Group Ib = conditioned in refrigerating HBO chamber for 12 hours (n=10); Group IIa = conditioned in cold storage for 24 hours (n=10); Group IIb = conditioned in refrigerating HBO chamber for 24 hours (n=10). After these preservation times, we studied intestinal tissue injury and morphometric analysis of the villi by H.E; apoptotic index by TUNEL and mitotic index by PCNA expression. Results: The grade of the lesions was significantly greater among group Ia and Ib compared to groups IIa and IIb, with smaller villi height and greater apoptotic index (p < 0,01). The apoptotic index was significantly smaller in group Ib compared to group IIb (p < o,01). The mitotic index was greater in segments preserved in hyperbaric chamber compared to conditioned in cold storage (p<0,05). Conclusion: The hyperbaric oxygenation protects the rat small bowel of the lesions in cold storage and preserves its structure adequately
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Citação
São Paulo: [s.n.], 2007. 84 p.