Paracoccidioides brasiliensis downmodulates α3 integrin levels in human lung epithelial cells in a TLR2-dependent manner

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dc.citation.volume10pt_BR
dc.contributor.authorBarros, Bianca Carla Silva Campitelli
dc.contributor.authorAlmeida, Bruna Rocha
dc.contributor.authorSuzuki, Erika [UNIFESP]
dc.contributor.authorLatteshttp://lattes.cnpq.br/3545915337077337pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/1449454874275064
dc.contributor.authorLatteshttp://lattes.cnpq.br/0670710968392309
dc.date.accessioned2023-04-27T18:18:58Z
dc.date.available2023-04-27T18:18:58Z
dc.date.issued2020-11-10
dc.description.abstractParacoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America and may be caused by the species Paracoccidioides brasiliensis. In the lungs, this fungus interacts with epithelial cells, activating host cell signalling pathways, resulting in the production of inflammatory mediators. This event may be initiated through the activation of Pattern-Recognition Receptors such as Toll-like Receptors (TLRs). By interacting with cell wall components, TLR2 is frequently related to fungal infections. In this work, we show that, after 24 h post-infection with P. brasiliensis, A549 lung epithelial cells presented higher TLR2 levels, which is important for IL-8 secretion. Besides, integrins may also participate in pathogen recognition by host cells. We verified that P. brasiliensis increased α3 integrin levels in A549 cells after 5 h of infection and promoted interaction between this receptor and TLR2. However, after 24 h, surprisingly, we verified a decrease of α3 integrin levels, which was dependent on direct contact between fungi and epithelial cells. Likewise, we observed that TLR2 is important to downmodulate α3 integrin levels after 24 h of infection. Thus, P. brasiliensis can modulate the host inflammatory response by exploiting host cell receptors and cell signalling pathways.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent19483pt_BR
dc.identifier.doihttps://doi.org/10.1093/mmy/myaa006pt_BR
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/67437
dc.languageengpt_BR
dc.publisherNaturept_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsinfo:eu-repo/semantics/openAccesspt_BR
dc.subjectIntegrinpt_BR
dc.subjectToll Like-receptorpt_BR
dc.subjectParacoccidoidespt_BR
dc.subjectEpithelial cellpt_BR
dc.titleParacoccidioides brasiliensis downmodulates α3 integrin levels in human lung epithelial cells in a TLR2-dependent mannerpt_BR
dc.typeinfo:eu-repo/semantics/articlept_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.departamentoMicrobiologia, Imunologia e Parasitologiapt_BR
unifesp.graduateProgramMicrobiologia e Imunologiapt_BR
unifesp.knowledgeAreaMicologiapt_BR
unifesp.researchAreaModulação de mecanismos celulares por Paracoccidioides em células epiteliais pulmonarespt_BR
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