Lineage-Negative Bone Marrow Cells Protect Against Chronic Renal Failure

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dc.contributor.authorAlexandre, Cristianne Silva
dc.contributor.authorVolpini, Rildo Aparecido
dc.contributor.authorShimizu, Maria Heloisa
dc.contributor.authorSanches, Talita Rojas
dc.contributor.authorSemedo, Patricia [UNIFESP]
dc.contributor.authorDi Jura, Vera Lucia
dc.contributor.authorCamara, Niels Olsen [UNIFESP]
dc.contributor.authorSeguro, Antonio Carlos
dc.contributor.authorAndrade, Lucia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:01Z
dc.date.available2016-01-24T13:52:01Z
dc.date.issued2009-01-01
dc.description.abstractProgressive renal failure continues to be a challenge. the use of bone marrow cells represents a means of meeting that challenge. We used lineage-negative (Lin(-)) cells to test the hypothesis that Lin(-) cell treatment decreases renal injury. Syngeneic Fischer 344 rats were divided into four groups: sham ( laparotomy only, untreated); Nx (five-sixth nephrectomy and untreated); NxLC1 (five-sixth nephrectomy and receiving 2 x 10(6) Lin(-) cells on postnephrectomy day 15); and NxLC3 (five-sixth nephrectomy and receiving 2 x 10(6) Lin(-) cells on postnephrectomy days 15, 30, and 45). On postoperative day 16, renal mRNA expression of interleukin (IL)-1 beta, tumor necrosis factor-alpha, and IL-6 was lower in NxLC rats than in Nx rats. On postnephrectomy day 60, NxLC rats presented less proteinuria, glomerulosclerosis, anemia, renal infiltration of immune cells, and protein expression of monocyte chemoattractant protein-1, as well as decreased interstitial area. Immunostaining for proliferating cell nuclear antigen showed that, in comparison with sham rats, Nx rats presented greater cell proliferation, whereas NxLC1 rats and NxLC3 rats presented less cell proliferation than did Nx rats. Protein expression of the cyclin-dependent kinase inhibitor p21 and of vascular endothelial growth factor increased after nephrectomy and decreased after Lin(-) cell treatment. On postnephrectomy day 120, renal function (inulin clearance) was significantly better in Lin(-) cell-treated rats than in untreated rats. Lin(-) cell treatment significantly improved survival. These data suggest that Lin(-) cell treatment protects against chronic renal failure. STEM CELLS 2009; 27: 682-692en
dc.description.affiliationUniv São Paulo, Sch Med, Fac Med,Lab Pesquisa Basica LIM 12, Dept Nephrol, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Nephrol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Immunol, BR-01246903 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Nephrol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent682-692
dc.identifierhttp://dx.doi.org/10.1634/stemcells.2008-0496
dc.identifier.citationStem Cells. Durham: Alphamed Press, v. 27, n. 3, p. 682-692, 2009.
dc.identifier.doi10.1634/stemcells.2008-0496
dc.identifier.issn1066-5099
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31128
dc.identifier.wosWOS:000264706900019
dc.language.isoeng
dc.publisherAlphamed Press
dc.relation.ispartofStem Cells
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHematopoietic stem cellsen
dc.subjectNephrectomyen
dc.subjectKidney failureen
dc.subjectChronic proteinuriaen
dc.subjectFibrosis/pathologyen
dc.subjectProliferating cell nuclear antigenen
dc.titleLineage-Negative Bone Marrow Cells Protect Against Chronic Renal Failureen
dc.typeinfo:eu-repo/semantics/article
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