Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil

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dc.citation.issue2
dc.citation.volume112
dc.contributor.authorVerza, Mirela
dc.contributor.authorSchmid, Karen Barros
dc.contributor.authorBarcellos, Regina Bones
dc.contributor.authorLinck, Natali
dc.contributor.authorBello, Graziele Lima
dc.contributor.authorScapin, Daniel
dc.contributor.authorSperhacke, Rosa Dea
dc.contributor.authorNunes Silva, Marcia Susana
dc.contributor.authorWollheim, Claudia
dc.contributor.authorCelle Rivero, Martha Gabriela [UNIFESP]
dc.contributor.authorKritski, Afranio Lineu
dc.contributor.authorRezende, Leonides, Jr.
dc.contributor.authorOliveira, Martha Maria
dc.contributor.authorDalla Costa, Elis Regina
dc.contributor.authorRosa Rossetti, Maria Lucia
dc.coverageRio De Janeiro, Rj
dc.date.accessioned2020-07-17T14:03:06Z
dc.date.available2020-07-17T14:03:06Z
dc.date.issued2017
dc.description.abstractBACKGROUND In high tuberculosis (TB) burden countries, there are few data on the performance of new molecular commercialised assays developed locally. OBJECTIVE To evaluate the performance of a new molecular commercialised assay for TB diagnosis (Detect-TB) in three laboratories. METHODS A total of 302 sputum samples from an equal number of patients with presumptive diagnosis of pulmonary tuberculosis (PTB) were submitted for routine smear microscopy, culture, and Detect-TB assay at three different sites in Brazil (the cities of Caxias do Sul, Sao Paulo and Canoas). FINDINGS Seventy four (24.7%) TB cases were diagnosed (65 bacteriologically confirmed). When compared to smear microscopy/ culture results, the overall sensitivity and specificity of Detect-TB assay was 84.6% (CI 95%en
dc.description.abstract73.7-91.6) and 93.1% (CI 95%en
dc.description.abstract89.195.8), respectively. When compared to bacteriological and clinical diagnostic criteria, the sensitivity and specificity of Detect-TB assay was 74.3% (CI 95%en
dc.description.abstract63.3-82.9) and 92.9% (CI 95%en
dc.description.abstract88.7-95.6), respectively. Among the three sites - Caxias do Sul, Sao Paulo and Canoas - the sensitivity and specificity were respectively 94.7% and 97.8%en
dc.description.abstract71.4% and 93.9%, 82.1% and 88.9%. MAIN CONCLUSIONS These findings suggest that the Detect-TB assay could be applied routinely in reference laboratories across different regions in Brazil.en
dc.description.affiliationFundacao Estadual Prod & Pesquisa Saude, Ctr Desenvolvimento Cient & Tecnol, Porto Alegre, RS, Brazil
dc.description.affiliationUniv Caxias Do Sul, Lab Pesquisa HIV AIDS, Caxias Do Sul, RS, Brazil
dc.description.affiliationUniv Caxias Do Sul, Lab Microbiol Clin, Caxias Do Sul, RS, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Lab Especial Microbiol Clin, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Fac Med, Ctr Pesquisa TB,Lab Genet & Biol Mol, Rio De Janeiro, RJ, Brazil
dc.description.affiliationLabtest, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Luterana Brasil, Canoas, RS, Brazil
dc.description.affiliationRede Brasileira Pesquisa TB, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Especial Microbiol Clin, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq/INCT
dc.description.sponsorshipFINEP
dc.description.sponsorshipLabtest
dc.description.sponsorshipFEPPS
dc.description.sponsorshipIDCNPq/INCT: 573548/2008-0
dc.format.extent94-99
dc.identifierhttp://dx.doi.org/10.1590/0074-02760160196
dc.identifier.citationMemorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj, v. 112, n. 2, p. 94-99, 2017.
dc.identifier.doi10.1590/0074-02760160196
dc.identifier.fileWOS000394155800002.pdf
dc.identifier.issn0074-0276
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55167
dc.identifier.wosWOS:000394155800002
dc.language.isoeng
dc.publisherFundaco Oswaldo Cruz
dc.relation.ispartofMemorias Do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecttuberculosisen
dc.subjectreverse-hybridisation assayen
dc.subjectIS6110en
dc.subjectmolecular diagnosisen
dc.subjectDetect-TBen
dc.titlePerformance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazilen
dc.typeinfo:eu-repo/semantics/article
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