Serum from children with polyarticular juvenile idiopathic arthritis (pJIA) inhibits differentiation, mineralization and may increase apoptosis of human osteoblasts in vitro

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dc.contributor.authorCaparbo, Valeria F.
dc.contributor.authorPrada, Flavia
dc.contributor.authorSilva, Clovis A. A.
dc.contributor.authorRegio, Paula L.
dc.contributor.authorPereira, Rosa M. R.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:03Z
dc.date.available2016-01-24T13:52:03Z
dc.date.issued2009-01-01
dc.description.abstractWe examined the effects of polyarticular juvenile idiopathic arthritis (pJIA) serum on proliferation, differentiation, mineralization, and apoptosis of human osteoblast cells (hOb) in culture. the hOb were cultured with 10% serum from active pJIA and healthy controls (CT) and were tested for DNA synthesis, alkaline phosphatase (AP) activity, osteocalcin (OC) secretion, calcium levels, caspase 3 activity, and DNA fragmentation. None of the patients had used glucocorticoids for at least 1 month before the study, or any other drug that can affect bone mineral metabolism. Human inflammatory cytokine levels (IL-6, IL-8, IL-10, IL-1 beta, TNF-alpha, and IL-12p70) were measured in pJIA and CT sera. Low levels of AP activity was observed in pJIA cultures compared with CT cultures (67.16 +/- 53.35 vs 100.11 +/- 50.64 mu mol p-nitrophenol/h(-1) mg(-1) protein, P=0.008). There was also a significant decrease in OC secretion (9.23 +/- 5.63 vs 12.82 +/- 7.02 ng/mg protein, P=0.012) and calcium levels (0.475 +/- 0.197 vs 0.717 +/- 0.366 mmol/l, P=0.05) in pJIA hOb cultures. No difference was observed in cell proliferation (323.56 +/- 108.23 vs 328.91 +/- 88.03 dpm/mg protein, P=0.788). Osteoblasts cultured with JIA sera showed lower levels of DNA and increased fragmentation than osteoblasts cultured with CT sera. pJIA sera showed higher IL-6 values than CT (21.44 +/- 9.31 vs 3.58 +/- 2.38 pg/ml, P<0.001), but no difference was observed related to IL-8, IL-10, IL-1 beta, TNF-alpha, and IL-12p70 between pJIA and controls. This study suggests that serum from children with pJIA inhibits differentiation, mineralization and may increase apoptosis of hOb cultures, and inflammatory cytokines such as IL-6 might be a mechanism in this find. These results may represent an alternative therapeutic target for prevention and treatment of bone loss in JIA.en
dc.description.affiliationUniv São Paulo, Fac Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Div Rheumatol, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Orthoped, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Pediat, BR-01246903 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPIBIC fellowship
dc.description.sponsorshipIDFAPESP: 01/13835-6
dc.description.sponsorshipIDPIBIC fellowship: 80.30.70/87.0
dc.format.extent71-77
dc.identifierhttp://dx.doi.org/10.1007/s10067-008-0985-y
dc.identifier.citationClinical Rheumatology. London: Springer London Ltd, v. 28, n. 1, p. 71-77, 2009.
dc.identifier.doi10.1007/s10067-008-0985-y
dc.identifier.issn0770-3198
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31156
dc.identifier.wosWOS:000261185900012
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofClinical Rheumatology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectApoptosisen
dc.subjectBoneen
dc.subjectDifferentiationen
dc.subjectJuvenile idiopathic arthritisen
dc.subjectMineralizationen
dc.subjectOsteoblasten
dc.titleSerum from children with polyarticular juvenile idiopathic arthritis (pJIA) inhibits differentiation, mineralization and may increase apoptosis of human osteoblasts in vitroen
dc.typeinfo:eu-repo/semantics/article
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