Glycosaminoglycan distribution in the rat uterine cervix during the estrous cycle

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dc.contributor.authorCubas, Jairo Jose Matozinho [UNIFESP]
dc.contributor.authorSimões, Ricardo Santos
dc.contributor.authorOliveira-Filho, Ricardo Martins
dc.contributor.authorSimões, Manuel de Jesus [UNIFESP]
dc.contributor.authorBaracat, Edmund Chada [UNIFESP]
dc.contributor.authorSoares-Junior, José Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-06-14T13:41:24Z
dc.date.available2015-06-14T13:41:24Z
dc.date.issued2010-01-01
dc.description.abstractOBJECTIVE: To analyze the amount of glycosaminoglycans in the uterine cervix during each phase of the rat estrous cycle. DESIGN: Based on vaginal smears, forty female, regularly cycling rats were divided into four groups (n = 10 for each group): GI - proestrous, GII - estrous, GIII - metaestrous and GIV - diestrous. Animals were sacrificed at each phase of the cycle, and the cervix was immediately removed and submitted to biochemical extraction and determination of sulfated glycosaminoglycans and hyaluronic acid. The results were analyzed by ANOVA followed by the Bonferroni post-hoc test. RESULTS: The uterine cervix had the highest amount of total sulfated glycosaminoglycans and dermatan sulfate during the estrous phase (8.90 ± 0.55 mg/g of cetonic extract, p<0.001; and 8.86 ± 0.57 mg/g of cetonic extract, p<0.001). In addition, there was more heparan sulfate at the cervix during the proestrous phase (0.185 ± 0.03 mg/g of cetonic extract) than during any other phase (p<0.001). There were no significant changes in the concentration of hyaluronic acid in the uterine cervix during the estrous cycle. CONCLUSION: Our data suggest that the amount of total sulfated glycosaminoglycans may be influenced by hormonal fluctuations related to the estrous cycle, with dermatan sulfate and heparan sulfate being the glycosaminoglycans most sensitive to hormonal change.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departmento de Morfologia e Genética
dc.description.affiliationUniversidade de São Paulo Faculdade de Medicina Departamento de Obstetrícia e Ginecologia
dc.description.affiliationUniversidade de São Paulo Instituto de Ciências Biomédicas Department of Pharmacology
dc.description.affiliationEscola Paulista de Medicina Department of Gynecology
dc.description.affiliationUnifespUNIFESP, Departmento de Morfologia e Genética
dc.description.affiliationUnifespEscola Paulista de Medicina Department of Gynecology
dc.description.sourceSciELO
dc.format.extent703-708
dc.identifierhttp://dx.doi.org/10.1590/S1807-59322010000700009
dc.identifier.citationClinics. Faculdade de Medicina / USP, v. 65, n. 7, p. 703-708, 2010.
dc.identifier.doi10.1590/S1807-59322010000700009
dc.identifier.fileS1807-59322010000700009.pdf
dc.identifier.issn1807-5932
dc.identifier.scieloS1807-59322010000700009
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/5481
dc.identifier.wosWOS:000280808400009
dc.language.isoeng
dc.publisherFaculdade de Medicina / USP
dc.relation.ispartofClinics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHeparan sulfateen
dc.subjectDermatan sulfateen
dc.subjectHyaluronic aciden
dc.subjectCervix uterien
dc.subjectEstrous cycleen
dc.titleGlycosaminoglycan distribution in the rat uterine cervix during the estrous cycleen
dc.typeinfo:eu-repo/semantics/article
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