Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?

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dc.citation.issue2
dc.citation.volume21
dc.contributor.authorFerreira, Cecília da Silva [UNIFESP]
dc.contributor.authorCarvalho, Kátia Cândido [UNIFESP]
dc.contributor.authorMaganhin, Carla Cristina [UNIFESP]
dc.contributor.authorPaiotti, Ana Paula Ribeiro [UNIFESP]
dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorSimões, Manuel de Jesus [UNIFESP]
dc.contributor.authorBaracat, Edmund Chada [UNIFESP]
dc.contributor.authorSoares Júnior, José Maria [UNIFESP]
dc.coverageDordrecht
dc.date.accessioned2020-10-30T18:46:35Z
dc.date.available2020-10-30T18:46:35Z
dc.date.issued2016
dc.description.abstractMelatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Ginecol, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Fac Med, Dept Obstet & Ginecol, Lab Struct & Mol Gynecol LIM 58,Discipline Gineco, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Patol, Lab Pathol Mol, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Morfol & Genet, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ginecol, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Fac Med, Dept Obstet & Ginecol, Lab Struct & Mol Gynecol LIM 58,Discipline Gineco, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Patol, Lab Pathol Mol, BR-01246923 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Morfol & Genet, BR-01246923 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipIDFAPESP: 09/51754-0
dc.description.sponsorshipIDCNPq: 137442/2009-2
dc.format.extent155-162
dc.identifierhttps://doi.org/10.1007/s10495-015-1195-0
dc.identifier.citationApoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016.
dc.identifier.doi10.1007/s10495-015-1195-0
dc.identifier.issn1360-8185
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58519
dc.identifier.wosWOS:000368176900004
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofApoptosis
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMelatoninen
dc.subjectFemale ratsen
dc.subjectContinuous lighten
dc.subjectApoptosisen
dc.titleDoes melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?en
dc.typeinfo:eu-repo/semantics/article
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