Immobilization of Amano lipase from Pseudomonas fluorescens on silk fibroin spheres: an alternative protocol for the enantioselective synthesis of halohydrins

dc.citation.issue21
dc.citation.volume7
dc.contributor.authorFerreira, Irlon Maciel [UNIFESP]
dc.contributor.authorYoshioka, Sergio A.
dc.contributor.authorComassetode, Joao V. [UNIFESP]
dc.contributor.authorPorto, Andre L. M.
dc.coverageCambridge
dc.date.accessioned2020-07-31T12:46:52Z
dc.date.available2020-07-31T12:46:52Z
dc.date.issued2017
dc.description.abstractThe search for a new, efficient, cheaper and sustainable matrix for lipase immobilization is a growing area in biotechnology. Amano lipase from Pseudomonas fluorescens was immobilized on silk fibroin spheres and used in the enzymatic kinetic resolution of halohydrins, to obtain optically active epoxides (up to 99% ee), important precursors in the synthesis of derivative antifungal azoles. This paper reinforces the versatility of silk fibroin as a support for heterogeneous catalysts.en
dc.description.affiliationUniv Fed Sao Carlos, Inst Quim Sao Carlos, Ave Joao Dagnone, Ed Quim Ambiental, BR-13563120 Sao Carlos, Brazil
dc.description.affiliationUniv Fed Amapa, Grp Biocatalise & Biotransformacao Quim Organ, Rod Juscelino Kubitscheck,KM 02 S-N, BR-68903419 Macapa, AP, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim Sao Carlos, Lab Bioquim & Biomat, Ave Trabalhador Sao Carlense 400, BR-13560970 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Ave Prof Artur Riedel 275, BR-09972270 Diadema, SP, Brazil
dc.description.affiliationUnifespInstituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Av. Prof. Artur Riedel, 275, 09972-270 Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCNPq: 558062/2009-1
dc.description.sponsorshipIDCNPq: 558062/2009-1
dc.description.sponsorshipIDFAPESP: 2009/50688-3
dc.format.extent12650-12658
dc.identifierhttp://dx.doi.org/10.1039/c7ra00083a
dc.identifier.citationRsc Advances. Cambridge, v. 7, n. 21, p. 12650-12658, 2017.
dc.identifier.doi10.1039/c7ra00083a
dc.identifier.fileWOS000395934500026.pdf
dc.identifier.issn2046-2069
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56425
dc.identifier.wosWOS:000395934500026
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofRsc Advances
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleImmobilization of Amano lipase from Pseudomonas fluorescens on silk fibroin spheres: an alternative protocol for the enantioselective synthesis of halohydrinsen
dc.typeinfo:eu-repo/semantics/article
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