Effects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic rat

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dc.contributor.authorKira, Vicente Massaji [UNIFESP]
dc.contributor.authorFagundes, Djalma José [UNIFESP]
dc.contributor.authorBandeira, César Orlando Peralta [UNIFESP]
dc.contributor.authorKaufman, Oskar [UNIFESP]
dc.contributor.authorFagundes, Anna T. N. [UNIFESP]
dc.contributor.authorOrtiz, Valdemar [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:38:23Z
dc.date.available2015-06-14T13:38:23Z
dc.date.issued2008-02-01
dc.description.abstractOBJECTIVE: To assess the effect of repeated extracorporeal shock waves (ESW) on the apoptosis of renal parenchyma in normal and diabetic rats by the caspase-3 expression. MATERIALS AND METHODS: 80 rats were assigned for ESW (Direx Tripter X1® - 14 KVA) to one of three groups with 50% rats with diabetes (A) and 50% normal (B) in each: G1 (n = 40): no ESW; G2 (n = 20): one ESW; G3 (n = 20): two ESWin an interval of 14 days. The animals were sacrificed 3 days after the ESW and samples of the renal parenchyma were histologically prepared, stained by the caspase-3 cleavage immunohistochemical method, and the apoptotic index (ApIn) was calculated by computer program Image Pro-Plus V 4,5,1 Media Cybernetics® ( ApIn = number of apoptotic cells in the microscopic section / total cells in the microscopic section). RESULTS: There was no statistical difference in ApIn of normal rats with one ESW (8.4 + 0.2) or two ESW (9.1 + 0.3) and both were similar to the control group with no ESW (7.9 + 0.2). The ApIn of diabetic rats with one ESW (7.4 + 0.3) was similar to the normal rats with one or two ESW. On the other hand, the ApIn was statistically higher in diabetic rats with two ESW (11.9 + 0.4) than in diabetic rats with one ESW (7.4 + 0.3). CONCLUSIONS: The ApIn of normal rats with a single (2,000 SW) or repeated ESW (4,000 SW) was similar to the control animals without any ESW. In diabetic rats, the repeated ESW (4,000 SW) may result in an additional risk of tissue damage expressed by the higher ApIn.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM) Department of Surgery
dc.description.affiliationUnifespUNIFESP, EPM, Department of Surgery
dc.description.sourceSciELO
dc.format.extent91-96
dc.identifierhttp://dx.doi.org/10.1590/S1677-55382008000100013
dc.identifier.citationInternational braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 1, p. 91-96, 2008.
dc.identifier.doi10.1590/S1677-55382008000100013
dc.identifier.fileS1677-55382008000100013.pdf
dc.identifier.issn1677-5538
dc.identifier.scieloS1677-55382008000100013
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/4221
dc.identifier.wosWOS:000207684600024
dc.language.isoeng
dc.publisherSociedade Brasileira de Urologia
dc.relation.ispartofInternational braz j urol
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectkidneyen
dc.subjectlithotripsyen
dc.subjecthigh-energy shock wavesen
dc.subjectdiabetesen
dc.subjectratsen
dc.subjectapoptosisen
dc.titleEffects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic raten
dc.typeinfo:eu-repo/semantics/article
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