Clotrimazole is highly effective in vitro against feline Sporothrix brasiliensis isolates

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dc.citation.issue11
dc.citation.volume66
dc.contributor.authorGagini, Thalita
dc.contributor.authorBorba-Santos, Luana Pereira
dc.contributor.authorRodrigues, Anderson Messias [UNIFESP]
dc.contributor.authorde Camargo, Zoilo Pires [UNIFESP]
dc.contributor.authorRozental, Sonia
dc.coverageLondon
dc.date.accessioned2020-09-01T13:21:27Z
dc.date.available2020-09-01T13:21:27Z
dc.date.issued2017
dc.description.abstractPurpose. Sporothrix brasiliensis, the most virulent species in the Sporothrix schenckii complex, is responsible for the ongoing epidemics of human and animal sporotrichosis in Brazil. Feline outbreaks are usually driven by S. brasiliensis and followed by extensive transmission to humans. Itraconazole is the first-line treatment for both feline and human sporotrichosis; however, reduced sensitivity is an emerging issue. Thus, we investigated the effect of the widely used antifungal clotrimazole - alone or in combination with itraconazole - against the pathogenic (yeast) form of feline and human S. brasiliensis isolates, in vitro. Methodology. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values were determined for treatment with clotrimazole and itraconazole, as monotherapy or in combination. In addition, the effect of the drugs on neutral lipid levels and the yeast ultrastructure were evaluated by flow cytometry and transmission electron microscopy (TEM), respectively. Results. The MIC and MFC values show that clotrimazole was more effective than itraconazole against feline S. brasiliensis isolates, while human isolates were more sensitive to itraconazole. Similarly to itraconazole, treatment with clotrimazole induced statistically significant neutral lipid accumulation in S. brasiliensis yeasts, and treated yeasts displayed irregularities in the cell membrane and a thicker cell wall when observed by TEM. Clotrimazole increased the antifungal activity of itraconazole in combination assays, with a synergistic effect for two feline isolates. Conclusion. The strong activity of clotrimazole against feline S. brasiliensis isolates suggests that this drug is potentially a new alternative for the treatment of feline sporotrichosis, alone or in combination with itraconazole.en
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Biophys Carlos Chagas Filho, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Cell Biol Div, Dept Microbiol Immunol & Parasitol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Cell Biol Div, Dept Microbiol Immunol & Parasitol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)
dc.format.extent1573-1580
dc.identifierhttp://dx.doi.org/10.1099/jmm.0.000608
dc.identifier.citationJournal Of Medical Microbiology. London, v. 66, n. 11, p. 1573-1580, 2017.
dc.identifier.doi10.1099/jmm.0.000608
dc.identifier.issn0022-2615
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58275
dc.identifier.wosWOS:000414369800007
dc.language.isoeng
dc.publisherMicrobiology Soc
dc.relation.ispartofJournal Of Medical Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectclotrimazoleen
dc.subjectSporothrix brasiliensisen
dc.subjectantifungal activityen
dc.subjectfeline sporotrichosisen
dc.subjectitraconazoleen
dc.titleClotrimazole is highly effective in vitro against feline Sporothrix brasiliensis isolatesen
dc.typeinfo:eu-repo/semantics/article
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