Posaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of cyp51A Mutations on Estimation of Epidemiological Cutoff Values

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dc.citation.issue4
dc.citation.volumev. 62
dc.contributor.authorEspinel-Ingroff, A.
dc.contributor.authorTurnidge, J.
dc.contributor.authorAlastruey-Izquierdo, A.
dc.contributor.authorDannaoui, E.
dc.contributor.authorGarcia-Effron, G.
dc.contributor.authorGuinea, J.
dc.contributor.authorKidd, S.
dc.contributor.authorPelaez, T.
dc.contributor.authorSanguinetti, M.
dc.contributor.authorMeletiadis, J.
dc.contributor.authorBotterel, F.
dc.contributor.authorBustamante, B.
dc.contributor.authorChen, Y. -C.
dc.contributor.authorChakrabarti, A.
dc.contributor.authorChowdhary, A.
dc.contributor.authorChryssanthou, E.
dc.contributor.authorCordoba, S.
dc.contributor.authorGonzalez, G. M.
dc.contributor.authorGuarro, J.
dc.contributor.authorJohnson, E. M.
dc.contributor.authorKus, J. V.
dc.contributor.authorLass-Floerl, C.
dc.contributor.authorLinares-Sicilia, M. J.
dc.contributor.authorMartin-Mazuelos, E.
dc.contributor.authorNegri, Clara Ezequiel [UNIFESP]
dc.contributor.authorPfaller, M. A.
dc.contributor.authorTortorano, A. M.
dc.coverageWashington
dc.date.accessioned2020-07-20T16:31:10Z
dc.date.available2020-07-20T16:31:10Z
dc.date.issued2018
dc.description.abstractEstimating epidemiological cutoff endpoints (ECVs/ECOFFS) may be hindered by the overlap of MICs for mutant and nonmutant strains (strains harboring or not harboring mutations, respectively). Posaconazole MIC distributions for the Aspergillus fumigatus species complex were collected from 26 laboratories (in Australia, Canada, Europe, India, South and North America, and Taiwan) and published studies. Distributions that fulfilled CLSI criteria were pooled and ECVs were estimated. The sensitivity of three ECV analytical techniques (the ECOFFinder, normalized resistance interpretation [NRI], derivatization methods) to the inclusion of MICs for mutants was examined for three susceptibility testing methods (the CLSI, EUCAST, and Etest methods). The totals of posaconazole MICs for nonmutant isolates (isolates with no known cyp51A mutations) and mutant A. fumigatus isolates were as follows: by the CLSI method, 2,223 and 274, respectivelyen
dc.description.abstractby the EUCAST method, 556 and 52, respectivelyen
dc.description.abstractand by Etest, 1,365 and 29, respectively. MICs for 381 isolates with unknown mutational status were also evaluated with the Sensititre Yeast-One system (SYO). We observed an overlap in posaconazole MICs among nonmutants and cyp51A mutants. At the commonly chosen percentage of the modeled wild-type population (97.5%), almost all ECVs remained the same when the MICs for nonmutant and mutant distributions were merged: ECOFFinder ECVs, 0.5 mu g/ml for the CLSI method and 0.25 mu g/ml for the EUCAST method and Etesten
dc.description.abstractNRI ECVs, 0.5 mu g/ml for all three methods. However, the ECOFFinder ECV for 95% of the nonmutant population by the CLSI method was 0.25 mu g/ml. The tentative ECOFFinder ECV with SYO was 0.06 mu g/ml (data from 3/8 laboratories). Derivatization ECVs with or without mutant inclusion were either 0.25 mu g/ml (CLSI, EUCAST, Etest) or 0.06 mu g/ml (SYO). It appears that ECV analytical techniques may not be vulnerable to overlap between presumptive wild-type isolates and cyp51A mutants when up to 11.6% of the estimated wild-type population includes mutants.en
dc.description.affiliationVCU Med Ctr, Richmond, VA 23298 USA
dc.description.affiliationUniv Adelaide, Adelaide, SA, Australia
dc.description.affiliationNatl Ctr Microbiol, Inst Salud Carlos III, Mycol Reference Lab, Majadahonda, Madrid, Spain
dc.description.affiliationUniv Paris 05, Hop Europeen Georges Pompidou, AP HP, Fac Med,Unite Parasitol Mycol,Serv Microbiol, Paris, France
dc.description.affiliationUniv Nacl Litoral, CONICET, CCT, Fac Bioquim Ciencias Biol,Lab Micol Diagnost Mol, Santa Fe, Argentina
dc.description.affiliationHosp Gen Univ Gregorio Maranon, Serv Microbiol Clin Enfermedades Infecciosas VIH, Madrid, Spain
dc.description.affiliationInst Invest Sanitaria Gregorio Maranon, Madrid, Spain
dc.description.affiliationNatl Mycol Reference Ctr, Microbiol & Infect Dis, SA Pathol, Adelaide, SA, Australia
dc.description.affiliationHosp Univ Cent Asturias, Serv Microbiol, Asturias, Spain
dc.description.affiliationUniv Cattolica Sacro Cuore, Inst Microbiol, Rome, Italy
dc.description.affiliationUniv Athens, Med Sch, Attikon Hosp, Clin Microbiol Lab, Athens, Greece
dc.description.affiliationCHU Henri Mondor, Dept Virol Bacteriol Hyg Parasitol Mycol, DHU VIC, Unite Parasitol Mycol, Creteil, France
dc.description.affiliationUniv Peruana Cayetano Heredia, Inst Med Trop Alexander von Humboldt, Lima, Peru
dc.description.affiliationNatl Taiwan Univ, Hosp & Coll Med, Dept Internal Med, Taipei, Taiwan
dc.description.affiliationPostgrad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh, India
dc.description.affiliationUniv Delhi, Vallabhbhai Patel Chest Inst, Dept Med Mycol, Delhi, India
dc.description.affiliationUniv Sjukhuset, Univ Lab Karolinska, Klin Mikrobiol, Stockholm, Sweden
dc.description.affiliationInst Nacl Enfermedades Infecciosas Dr CG Malbran, Buenos Aires, DF, Argentina
dc.description.affiliationUniv Autonoma Nuevo Leon, Monterrey, Nuevo Leon, Mexico
dc.description.affiliationUniv Rovira & Virgili, Med Sch, Mycol Unit, Reus, Spain
dc.description.affiliationPubl Hlth England, Mycol Reference Lab, Bristol, Avon, England
dc.description.affiliationPubl Hlth Ontario, Toronto, ON, Canada
dc.description.affiliationMed Univ Innsbruck, Natl Mycol Reference Ctr, Div Hyg & Med Microbiol, Innsbruck, Austria
dc.description.affiliationUniv Cordoba, HGU Reina Sofia, Cordoba, Spain
dc.description.affiliationHosp Valme, Unidad Gest Clin Enfermedades Infecciosas & Micro, Seville, Spain
dc.description.affiliationUniv Fed Sao Paulo, Lab Especial Micol, Sao Paulo, Brazil
dc.description.affiliationUniv Iowa, Coll Med, Iowa City, IA USA
dc.description.affiliationUniv Milan, Dept Biomed Sci Hlth, Milan, Italy
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Especial Micol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1128/AAC.01916-17
dc.identifier.citationAntimicrobial Agents And Chemotherapy. Washington, v. 62, n. 4, 2018.
dc.identifier.doi10.1128/AAC.01916-17
dc.identifier.issn0066-4804
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55763
dc.identifier.wosWOS:000428392100026
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofAntimicrobial Agents And Chemotherapy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAspergillus fumigatusen
dc.subjectCLSI ECVsen
dc.subjectECVsen
dc.subjectEUCAST ECVsen
dc.subjectEtesten
dc.subjectSYOen
dc.subjectcyp51A mutantsen
dc.subjectposaconazoleen
dc.subjecttriazole resistanceen
dc.subjectwild typeen
dc.titlePosaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of cyp51A Mutations on Estimation of Epidemiological Cutoff Valuesen
dc.typeinfo:eu-repo/semantics/article
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