Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats

Página do item simplificado
dc.contributor.authorRomariz, Simone Amaro Alves [UNIFESP]
dc.contributor.authorPaiva, Daisylea de Souza [UNIFESP]
dc.contributor.authorValente, Maria Fernanda [UNIFESP]
dc.contributor.authorBarnabe, Gabriela Filoso [UNIFESP]
dc.contributor.authorFrussa-Filho, Roberto [UNIFESP]
dc.contributor.authorSilva, Regina Cláudia Barbosa da [UNIFESP]
dc.contributor.authorCalcagnotto, Maria Elisa
dc.contributor.authorLongo, Beatriz Monteiro [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Rio Grande do Sul
dc.date.accessioned2016-01-24T14:37:42Z
dc.date.available2016-01-24T14:37:42Z
dc.date.issued2014-08-02
dc.description.abstractBackground: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUNIFESP, Dept Biociencias, BR-11015020 Santos, SP, Brazil
dc.description.affiliationUniv Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Biociencias, BR-11015020 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.format.extent8
dc.identifierhttps://dx.doi.org/10.1186/1471-2202-15-94
dc.identifier.citationBmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014.
dc.identifier.doi10.1186/1471-2202-15-94
dc.identifier.fileWOS000341598500001.pdf
dc.identifier.issn1471-2202
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/38077
dc.identifier.wosWOS:000341598500001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Neuroscience
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnxietyen
dc.subjectMedial ganglionic eminenceen
dc.subjectNeuronal precursor cellsen
dc.subjectTransplantationen
dc.titleLong-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn ratsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000341598500001.pdf
Tamanho:
925.16 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos