Analysis of male reproductive parameters in a murine model of mucopolysaccharidosis type I (MPS I)

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dc.contributor.authorNascimento, Cinthia Castro do [UNIFESP]
dc.contributor.authorAguiar, Odair [UNIFESP]
dc.contributor.authorD'Almeida, Vania [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T17:44:19Z
dc.date.available2018-06-15T17:44:19Z
dc.date.issued2014-01-01
dc.description.abstractMucopolysaccharidosis (MPS) I is a lysosomal storage disorder (LSD) that is characterised by alpha-L-idu-ronidase (Idua) deficiency and continuous deposition of glycosaminoglycans (GAGs), which consequently interferes with cell signalling mechanisms and results in multisystemic and progressive symptoms. The animal model of MPS I (Idua-/-) has been widely studied to elucidate the consequences and progression of the disorder; however, studies specifically assessing the male reproductive tract are lacking. The aim of this study was to evaluate some of the reproductive characteristics of male MPS I mice in two phases of life. Reproductive organ biometry, sperm counts, sperm morphological evaluation, plasma testosterone measurements and histopathological, histomorphometrical and immunohistochemical analysis were performed in 3- and 6-month-old C57BL/6 Idua+/+ and Idua-/- mice. Seminal vesicle weights were decreased in both the 3- and 6-month-old Idua-/- mice. Decrease in sperm counts and the majority of the histopathological signs were observed in the 6-month-old Idua-/- mice. No differences were detected in the sperm morphological analysis. Immunohistochemistry revealed that seminiferous tubules from 3-month-old Idua-/- mice were more intensely stained with anti-caspase-3 than 3-month-old Idua+/+ mice, but no difference was found at 6 months. These results suggest that MPS I interferes with male reproductive parameters both in 3 and 6-month-old animals and histopathological signs are more pronounced in 6-month-old mice, indicating that the effects of the disorder may intensify with the disease progression.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipAFIP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent3488-3497
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097293/
dc.identifier.citationInternational Journal Of Clinical And Experimental Pathology. Madison: E-century Publishing Corp, v. 7, n. 6, p. 3488-3497, 2014.
dc.identifier.fileWOS000338770500089.pdf
dc.identifier.issn1936-2625
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/44034
dc.identifier.wosWOS:000338770500089
dc.language.isoeng
dc.publisherE-century Publishing Corp
dc.relation.ispartofInternational Journal Of Clinical And Experimental Pathology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectKnockout miceen
dc.subjectMucopolysaccharidosisen
dc.subjectSpermatogenesisen
dc.subjectTestisen
dc.titleAnalysis of male reproductive parameters in a murine model of mucopolysaccharidosis type I (MPS I)en
dc.typeinfo:eu-repo/semantics/article
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