Collagen XVIII/endostatin expression in experimental endotoxemic acute renal failure

Página do item simplificado
dc.contributor.authorCichy, Milene Cristina [UNIFESP]
dc.contributor.authorRocha, Flavia Gomes de Goes [UNIFESP]
dc.contributor.authorTristão, Vivian Regina [UNIFESP]
dc.contributor.authorPessoa, Edson de Andrade [UNIFESP]
dc.contributor.authorCenedeze, Marcos Antonio [UNIFESP]
dc.contributor.authorNürmberg Junior, R.
dc.contributor.authorSchor, Nestor [UNIFESP]
dc.contributor.authorBellini, Maria Helena [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInstituto de Pesquisas Energéticas e Nucleares Centro de Biotecnologia
dc.contributor.institutionFaculdades Metropolitanas Unidas Faculdade de Medicina Veterinária
dc.date.accessioned2015-06-14T13:41:17Z
dc.date.available2015-06-14T13:41:17Z
dc.date.issued2009-12-01
dc.description.abstractAcute renal failure (ARF) is a frequent complication of Gram-negative sepsis, with a high risk of mortality. Lipopolysaccharide (LPS)-induced ARF is associated with hemodynamic changes that are strongly influenced by the overproduction of nitric oxide (NO) through the cytokine-mediated up-regulation of inducible NO synthase. LPS-induced reductions in systemic vascular resistance paradoxically culminate in renal vasoconstriction. Collagen XVIII is an important component of the extracellular matrix expressed in basement membranes. Its degradation by matrix metalloproteases, cathepsins and elastases results in the formation of endostatin, claimed to have antiangiogenic activity and to be a prominent vasorelaxing agent. We evaluated the expression of endostatin/collagen XVIII in an endotoxemic ARF model. ARF was induced in C57BL/6 mice by intraperitoneal injection of LPS (10 mg/kg) followed by sacrifice 4 and 12 h later. Kidney tissue was the source of RNA and protein and the subject of histological analysis. As early as 4 h after LPS administration, blood urea, creatinine and NO levels were significantly increased compared to control. Endostatin/collagen XVIII mRNA levels were 0.71 times lower than sham-inoculated mice 4 h after LPS inoculation, returning to normal levels 12 h after LPS inoculation. Immunohistological examination revealed that acute injury caused by LPS leads to an increase of endostatin basement membrane staining in association with the decrease of CD31 endothelial basement membrane staining. These results indicate that in the early phase of endotoxemic ARF the endostatin levels were not regulated by gene expression, but by the metabolism of collagen XVIII.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Medicina (Nefrologia)
dc.description.affiliationInstituto de Pesquisas Energéticas e Nucleares Centro de Biotecnologia
dc.description.affiliationFaculdades Metropolitanas Unidas Faculdade de Medicina Veterinária
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Medicina (Nefrologia)
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2007/51204-4
dc.description.sponsorshipIDFAPESP: 2008/52652-3
dc.description.sponsorshipIDFAPESP: 2008/52654
dc.description.sponsorshipIDFAPESP: 2008/52613-8-6
dc.format.extent1150-1155
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2009007500007
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 12, p. 1150-1155, 2009.
dc.identifier.doi10.1590/S0100-879X2009007500007
dc.identifier.fileS0100-879X2009001200005.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2009001200005
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/5357
dc.identifier.wosWOS:000272487500005
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectAcute renal failureen
dc.subjectLipopolysaccharideen
dc.subjectGene expressionen
dc.subjectEndostatinen
dc.subjectCD31en
dc.titleCollagen XVIII/endostatin expression in experimental endotoxemic acute renal failureen
dc.typeArtigo
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0100-879X2009001200005.pdf
Tamanho:
1.61 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos