Apoptotic effect of ethanol is potentiated by caffeine-induced calcium release in rat astrocytes

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dc.contributor.authorHirata, H.
dc.contributor.authorMachado, L. S.
dc.contributor.authorOkuno, C. S.
dc.contributor.authorBrasolin, A.
dc.contributor.authorLopes, G. S.
dc.contributor.authorSmaili, Soraya Soubhi [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:40:55Z
dc.date.available2016-01-24T12:40:55Z
dc.date.issued2006-01-30
dc.description.abstractIn this study, we investigated agents that increased intracellular calcium levels and their correlation with apoptotic cell death induction. We used rat astrocytes to investigate the increase in cytosolic Ca2+, (Ca-c(2+)) and apoptosis induction by drugs that mobilize Ca2+ from different sources. We observed that thapsigargin (Thap), caffeine (Caff) and FCCP which caused similar increases in Ca-c(2+) levels (30-40%), also induced similar apoptotic rates (30-35%). On the other hand, antimycin (Anti), staurosporine (STS) and ethanol (Eth) promoted higher increases in Ca-c(2+) (55-65 %) and higher apoptotic rates (55-85%). Eth induced cell death in a concentration- and time-dependent manner. After treatment with Eth plus Caff for 6, 12 and 24 h, these effects were strongly potentiated. Results suggest that there might be a correlation between Ca-c(2+) increase and the rate of apoptosis. It is possible that Eth induces cell death by activation of more than one pathway and Ca-c(2+) might be one of the elements involved. the present work indicates that Ca-c(2+) can potentiate death by ethanol in rat astrocytes. (c) 2005 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Sch Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Sch Med, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent136-140
dc.identifierhttp://dx.doi.org/10.1016/j.neulet.2005.09.066
dc.identifier.citationNeuroscience Letters. Clare: Elsevier B.V., v. 393, n. 2-3, p. 136-140, 2006.
dc.identifier.doi10.1016/j.neulet.2005.09.066
dc.identifier.issn0304-3940
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28695
dc.identifier.wosWOS:000234583000012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNeuroscience Letters
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectethanolen
dc.subjectcalciumen
dc.subjectmitochondriaen
dc.subjectapoptosisen
dc.subjectastrocytesen
dc.subjectendoplasmic reticulumen
dc.titleApoptotic effect of ethanol is potentiated by caffeine-induced calcium release in rat astrocytesen
dc.typeinfo:eu-repo/semantics/article
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