Impact of adrenalectomy and dexamethasone treatment on testicular morphology and sperm parameters in rats: insights into the adrenal control of male reproduction

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dc.contributor.authorSilva, Erick José Ramo da [UNIFESP]
dc.contributor.authorVendramini, Vanessa [UNIFESP]
dc.contributor.authorRestelli, Adriana Ester [UNIFESP]
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.authorKempinas, Wilma De Grava
dc.contributor.authorAvellar, Maria Christina Werneck [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:38:03Z
dc.date.available2016-01-24T14:38:03Z
dc.date.issued2014-11-01
dc.description.abstractHere we investigated the hypothesis that normal levels of glucocorticoids, a class of adrenal steroid hormones, are required for normal testicular and epididymal functions. We examined the effects of the manipulation of glucocorticoid plasma levels by bilateral adrenalectomy (1, 2, 7 and 15days) alone or in combination with daily treatment with the synthetic glucocorticoid dexamethasone (DEX; 5g/kg, i.p., 6days) on the morphology of the testis and sperm parameters in rats. We showed that adrenalectomy led to a reduction in testicular sperm count and daily sperm production starting 2days after surgery and a differential decrease in sperm count in the epididymis, according to the region and time post-adrenalectomy analysed. in parallel, testes from 7-day adrenalectomized (ADX) rats displayed a higher frequency of damaged seminiferous tubules and the presence of elongated spermatids retained in the basal epithelial compartment in stages IX-XVII, which is indicative of defective spermiation. the alkaline comet assay revealed a late effect of adrenalectomy on epididymal sperm DNA fragmentation, which was increased only 15days after surgery. DEX treatment prevented the changes in testicular and epididymal sperm count observed in 7-day ADX rats, but failed to protect the testis from ADX-induced morphological abnormalities. Thus, our results indicated that glucocorticoids may be involved in events related to the maintenance of spermatogenesis and sperm maturation during adulthood. These findings provide new insights into the importance of adrenal steroids to male fertility.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Surg, Human Reprod Sect,Div Urol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, Botucatu, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Surg, Human Reprod Sect,Div Urol, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipFogarty International Center Program for Training and Research in Population and Health, USA
dc.description.sponsorshipUniversidade Federal de São Paulo/University of North Carolina at Chapel Hill
dc.description.sponsorshipIDFogarty International Center Program for Training and Research in Population and Health, USA: D43TW/HD00627
dc.format.extent835-846
dc.identifierhttps://dx.doi.org/10.1111/j.2047-2927.2014.00228.x
dc.identifier.citationAndrology. Hoboken: Wiley-Blackwell, v. 2, n. 6, p. 835-846, 2014.
dc.identifier.doi10.1111/j.2047-2927.2014.00228.x
dc.identifier.issn2047-2919
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/38365
dc.identifier.wosWOS:000344328700005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofAndrology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectAdrenalectomyen
dc.subjectAnimal modelsen
dc.subjectEpididymisen
dc.subjectHormonesen
dc.subjectRaten
dc.subjectSperm quality parametersen
dc.subjectTestisen
dc.titleImpact of adrenalectomy and dexamethasone treatment on testicular morphology and sperm parameters in rats: insights into the adrenal control of male reproductionen
dc.typeinfo:eu-repo/semantics/article
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