Mesenchymal stem cells from sternum: the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kinetics

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dc.citation.volume15]
dc.contributor.authorDias, Lucinara Dadda
dc.contributor.authorCasali, Karina Rabello [UNIFESP]
dc.contributor.authorGhem, Carine
dc.contributor.authorda Silva, Melissa Kristocheck
dc.contributor.authorSausen, Grasiele
dc.contributor.authorPalma, Patricia Bonini
dc.contributor.authorCovas, Dimas Tadeu
dc.contributor.authorKalil, Renato A. K.
dc.contributor.authorSchaan, Beatriz D.
dc.contributor.authorNardi, Nance Beyer
dc.contributor.authorMarkoski, Melissa Medeiros
dc.coverageLondon
dc.date.accessioned2020-06-26T16:30:14Z
dc.date.available2020-06-26T16:30:14Z
dc.date.issued2017
dc.description.abstractBackground: In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results. Methods: To study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases. We included patients with IHD (n = 42) or VHD (n = 20), with average age of 60 years and no differences in cardiovascular risk factors. BM samples were collected (16.4 +/- 6 mL) and submitted to centrifugation with Ficoll-Paque, yielding 4.5 +/- 1.5 x 10(7) cells/mL. Results: Morphology, immunophenotype and differentiation ability had proven that the cultivated sternal BM cells had MSC features. The colony forming unit-fibroblast (CFU-F) frequency was similar between groups (p = 0.510), but VHD samples showed positive correlation to plated cells vs. CFU-F number (r = 0.499, p = 0.049). The MSC culture was established in 29% of collected samples, achieved passage 9, without significant difference in expansion kinetics between groups (p > 0.05). Dyslipidemia and the use of statins was associated with culture establishment for IHD patients (p = 0.049 and p = 0.006, respectively). Conclusions: Together, these results show that the sternum bone can be used as a source for MSC isolation, and that ischemic or valvular diseases do not influence the cellular yield, culture establishment or in vitro growth kinetics.en
dc.description.affiliationFundacao Univ Cardiol, Inst Cardiol, Programa Posgrad Ciencias Saude Cardiol, Ave Princesa Isabel,370,3 Andar, BR-90620001 Porto Alegre, RS, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationHosp Clin Porto Alegre, Serv Patol Clin, Porto Alegre, RS, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Med Ribeirao Preto, Lab Citometria Fluxo, Ctr Reg Hemoterapia,Hosp Clin, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Ciencias Saude Porto Alegre, Porto Alegre, RS, Brazil
dc.description.affiliationUniv Fed Rio Grande do Sul, Fac Med, Porto Alegre, RS, Brazil
dc.description.affiliationUniv Luterana Brasil, Lab Celulas Tronco & Engn Tecidos, Canoas, RS, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Sao Jose Dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq-Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.description.sponsorshipCAPES-Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/Programa de Cooperacao Academico (Procad)
dc.description.sponsorshipFAPERGS-Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul
dc.description.sponsorshipIDCNPq
dc.description.sponsorshipIDCAPES
dc.description.sponsorshipIDFAPERGS
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s12967-017-1262-0]
dc.identifier.citationJournal Of Translational Medicine. London, v. 15, p. -, 2017.
dc.identifier.doi10.1186/s12967-017-1262-0
dc.identifier.fileWOS000406324500001.pdf
dc.identifier.issn1479-5876
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53434
dc.identifier.wosWOS:000406324500001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofJournal Of Translational Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMSC establishmenten
dc.subjectStem cells cultureen
dc.subjectCell therapyen
dc.subjectValvular heart diseaseen
dc.subjectIschemic heart diseaseen
dc.titleMesenchymal stem cells from sternum: the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kineticsen
dc.typeinfo:eu-repo/semantics/article
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