Effect of estrogen on muscarinic acetylcholine receptor expression in rat myometrium

Página do item simplificado
dc.contributor.authorAbdalla, FMF
dc.contributor.authorMarostica, E.
dc.contributor.authorPicarelli, Z. P.
dc.contributor.authorAbreu, L. C.
dc.contributor.authorAvellar, MCW
dc.contributor.authorPorto, C. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Butantan
dc.date.accessioned2016-01-24T12:34:14Z
dc.date.available2016-01-24T12:34:14Z
dc.date.issued2004-01-15
dc.description.abstractWe report the effect of acute estrogen treatment in the expression of muscarinic acetylcholine receptors (mAChRs) in myometrium. Strips were obtained from rats in estrus (control) and treated with estrogen, 24 It before the experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed and m2, m3 and m5 mAChR mRNA subtypes were detected in myometrium from both groups. [H-3]Quinuclidinyl benzilate ([(3)HQNB]) binding studies indicated that estrogen treatment did not change the affinity and density of mAChRs in myometrial membranes. Displacement curves of [(3)HQNB] with different mAChRs antagonists indicated a one-site fit for all antagonists tested. Comparison of pK(i) values indicated a significant correlation to M-2-mAChR subtype. Functional studies, however, showed that estrogen treatment increased myometrium sensitivity to carbachol and the calculated apparent affinity values were significantly correlated to M-3-mAChR. Furthermore, the pharmacological profile of the two populations of mAChR was not affected by estrogen. in conclusion, these results provide evidence for the presence of M-2- and M-3-mAChR, at the mRNA and protein level, in the rat myometrium and indicate that estrogen induces an increase in myometrial responsiveness to mAChR agonists. (C) 2003 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationInst Butantan, Pharmacol Lab, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent139-148
dc.identifierhttp://dx.doi.org/10.1016/j.mce.2003.10.040
dc.identifier.citationMolecular and Cellular Endocrinology. Clare: Elsevier Sci Ireland Ltd, v. 213, n. 2, p. 139-148, 2004.
dc.identifier.doi10.1016/j.mce.2003.10.040
dc.identifier.issn0303-7207
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27582
dc.identifier.wosWOS:000189308800004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectmuscarinic acetylcholine receptorsen
dc.subjectmyometriumen
dc.subjectestrogenen
dc.subjectcarbacholen
dc.titleEffect of estrogen on muscarinic acetylcholine receptor expression in rat myometriumen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral