Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates

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dc.contributor.authorRocha, Leticia B.
dc.contributor.authorOzaki, Christiane Y.
dc.contributor.authorHorton, Denise S. P. Q.
dc.contributor.authorMenezes, Caroline A.
dc.contributor.authorSilva, Anderson
dc.contributor.authorFernandes, Irene
dc.contributor.authorMagnoli, Fabio C.
dc.contributor.authorVaz, Tania M. I.
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.authorPiazza, Roxane M. F.
dc.contributor.institutionButantan Inst
dc.contributor.institutionSão Paulo Trop Med Inst
dc.contributor.institutionFleury Med & Hlth
dc.contributor.institutionAdolfo Lutz Inst
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:34:46Z
dc.date.available2016-01-24T14:34:46Z
dc.date.issued2013-12-01
dc.description.abstractEnterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one or a few ETEC isolates to define the production and release of these toxins, which hinders the detection of ETEC by phenotypic approaches. Thus, the present study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. Accordingly, a collection of 90 LT-, ST-, and ST/LT-producing ETEC isolates was used to determine a protocol for toxin production and release aimed at ETEC detection. for this, we used previously raised anti-LT antibodies and the anti-ST monoclonal and polyclonal antibodies described herein. the presence of bile salts and the use of certain antibiotics improved ETEC toxin production/release. Triton X-100, as chemical treatment, proved to be an alternative method for toxin release. Consequently, a common protocol that can increase the production and release of LT and ST toxins could facilitate and enhance the sensitivity of diagnostic tests for ETEC using the raised and described antibodies in the present work.en
dc.description.affiliationButantan Inst, Bacteriol Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationSão Paulo Trop Med Inst, Seroepidemiol & Immunol Lab, BR-05403000 São Paulo, Brazil
dc.description.affiliationFleury Med & Hlth, BR-04344903 São Paulo, Brazil
dc.description.affiliationButantan Inst, Immunopathol Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationButantan Inst, Immunochem Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationAdolfo Lutz Inst, Bacteriol Sect, BR-01246000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, BR-04923062 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, BR-04923062 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent2384-2402
dc.identifierhttp://dx.doi.org/10.3390/toxins5122384
dc.identifier.citationToxins. Basel: Mdpi Ag, v. 5, n. 12, p. 2384-2402, 2013.
dc.identifier.doi10.3390/toxins5122384
dc.identifier.fileWOS000332792300007.pdf
dc.identifier.issn2072-6651
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37019
dc.identifier.wosWOS:000332792300007
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofToxins
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectETECen
dc.subjectheat-labile toxinen
dc.subjectheat-stable toxinen
dc.subjectproductionen
dc.subjectreleaseen
dc.subjectdetectionen
dc.titleDifferent Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolatesen
dc.typeinfo:eu-repo/semantics/article
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