Osteoconductive properties of two different bioactive glass forms (powder and fiber) combined with collagen

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dc.citation.volume423
dc.contributor.authorMagri, Angela Maria Paiva [UNIFESP]
dc.contributor.authorFernandes, Kelly Rossetti [UNIFESP]
dc.contributor.authorUeno, Fabio Roberto [UNIFESP]
dc.contributor.authorKido, Hueliton Wilian [UNIFESP]
dc.contributor.authorSilva, Antonio Carlos da
dc.contributor.authorBraga, Francisco José Correa
dc.contributor.authorGranito, Renata Neves [UNIFESP]
dc.contributor.authorGabbai-Armelin, Paulo Roberto [UNIFESP]
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-09-01T13:21:15Z
dc.date.available2020-09-01T13:21:15Z
dc.date.issued2017
dc.description.abstractBioactive Glasses (BG) is a group of synthetic silica-based materials with the unique ability to bond to living bone and can be used in bone repair. Although the osteogenic potential of BG, this material may have not present sufficient osteoconductive and osteoinductive properties to allow bone regeneration, especially in compromised situations. In order to overcome this limitation, it was proposed the combination the BG in two forms (powder and fiber) combined with collagen type I (COL-1). The aim of this study was to evaluate the BG/COL-based materials in terms of morphological characteristics, physicochemical features and mineralization. Additionally, the second objective was to investigate and compare the osteoconductive properties of two different bioactive glass forms (powder and fiber) enriched or not with collagen using a tibial bone defect model in rats. For this, four different formulations (BG powder -BGp, BG powder enriched with collagen - BGp/Col, BG fibers - BGf and BGp fibers enriched with collagen - BGf/Col) were developed. The physicochemical and morphological modifications were analyzed by SEM, FTIR, calcium assay and pH measurement. For in vivo evaluations, histopathology, morphometrical and immunohistochemistry were performed in a tibial defect in rats. The FTIR analysis indicated that BGp and BGf maintained the characteristic peaks for this class of material. Furthermore, the calcium assay showed an increased Ca uptake in the BG fibers. The pH measurements revealed that BGp (with or without collagen) presented higher pH values compared to BGf. In addition, the histological analysis demonstrated no inflammation for all groups at the site of the injury, besides a faster material degradation and higher bone ingrowth for groups with collagen. The immunohistochemistry analysis demonstrated Runx-2 and Rank-L expression for all the groups. Those findings support that BGp with collagen can be a promising alternative for treating fracture of difficult consolidation. (C) 2017 Elsevier B.V. All rights reserved.en
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Nucl & Energy Res Inst IPEN, Ave Lineu Prestes 2242, BR-05508000 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent557-565
dc.identifierhttps://dx.doi.org/10.1016/j.apsusc.2017.06.152
dc.identifier.citationApplied Surface Science. Amsterdam, v. 423, p. 557-565, 2017.
dc.identifier.doi10.1016/j.apsusc.2017.06.152
dc.identifier.issn0169-4332
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58158
dc.identifier.wosWOS:000410607500066
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofApplied Surface Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBioactive glassesen
dc.subjectCollagenen
dc.subjectCompositeen
dc.subjectFibersen
dc.subjectPowderen
dc.subjectBone repairen
dc.titleOsteoconductive properties of two different bioactive glass forms (powder and fiber) combined with collagenen
dc.typeinfo:eu-repo/semantics/article
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