Low-intensity pulsed ultrasound-dependent osteoblast proliferation occurs by via activation of the P2Y receptor: Role of the P2Y(1) receptor

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dc.contributor.authorAlvarenga, Erika Costa [UNIFESP]
dc.contributor.authorRodrigues, Renata [UNIFESP]
dc.contributor.authorCaricati-Neto, Afonso [UNIFESP]
dc.contributor.authorSilva-Filho, Fernando Costa
dc.contributor.authorParedes-Gamero, Edgar J. [UNIFESP]
dc.contributor.authorFerreira, Alice T. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2016-01-24T13:59:16Z
dc.date.available2016-01-24T13:59:16Z
dc.date.issued2010-02-01
dc.description.abstractLow-intensity pulsed ultrasound (LIPUS) is commonly used in the treatment of fractures and nonunion-promoting acceleration of healing fractures. in this report, we investigated the implication of the P2 receptors in osteoblast proliferation induced with LIPUS treatment. We observed that ADP, ATP, UTP, and UDP promote osteoblast increase and an increase of intracellular Ca2+, through activation of P2Y receptors. Osteoblasts' expression of the P2Y(1), P2Y(2), P2Y(4), P2Y(6), P2Y(11), P2Y(12), and P2Y(13) receptors was confirmed. in addition, the participation of the P2Y(1) receptor in osteoblast increase and the ADP-dependent increase of Ca2+ concentration were shown. Furthermore, release of ATP/purines was induced by LIPUS treatment. Finally, LIPUS-dependent osteoblast increase was abolished in the presence of the Ca2+ chelator (BAPTA), the inositol 1,4,5-trisphosphate receptor antagonist (2-APB), and the selective P2Y(1) receptor antagonist (MRS2179). in conclusion, LIPUS treatment induces osteoblastogenesis via the release of purines, such as ATP, activating P2Y receptors, mainly the P2Y(1) receptor. (C) 2009 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Dept Biofis, Inst Biofis Carlos Chagas Filho, BR-21941 Rio de Janeiro, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent355-362
dc.identifierhttp://dx.doi.org/10.1016/j.bone.2009.09.017
dc.identifier.citationBone. New York: Elsevier B.V., v. 46, n. 2, p. 355-362, 2010.
dc.identifier.doi10.1016/j.bone.2009.09.017
dc.identifier.issn8756-3282
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32218
dc.identifier.wosWOS:000274702800016
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBone
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectOsteoblastsen
dc.subjectUltrasounden
dc.subjectP2 receptorsen
dc.subjectATPen
dc.subjectProliferationen
dc.subjectCalciumen
dc.titleLow-intensity pulsed ultrasound-dependent osteoblast proliferation occurs by via activation of the P2Y receptor: Role of the P2Y(1) receptoren
dc.typeArtigo
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