Food restriction and refeeding induces changes in lipid pathways and fat deposition in the adipose and hepatic tissues in rats with diet-induced obesity

Página do item simplificado
dc.contributor.authorDuarte, Fernanda Oliveira
dc.contributor.authorSene-Fiorese, Marcela
dc.contributor.authorCheik, Nadia Carla
dc.contributor.authorMaria, Adriana Simone Lopes Santa
dc.contributor.authorAquino, Antonio Eduardo de
dc.contributor.authorOishi, Jorge Camargo
dc.contributor.authorRossi, Elizeu Antonio
dc.contributor.authorDuarte, Ana Claudia Garcia de Oliveira
dc.contributor.authorDâmaso, Ana Raimunda [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniv Estadual Paulista
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:23Z
dc.date.available2016-01-24T14:27:23Z
dc.date.issued2012-07-01
dc.description.abstractThe aim of this study was to determine the effects of successive cycles of a moderately restrictive diet and refeeding with a high-fat diet on the metabolism of the adipose and hepatic tissues of obese rats. Rats were assigned to the following groups: a chow diet; a high-fat diet; a moderate caloric restriction; or a moderate caloric restriction plus refeeding. Some animals in each group were given [1-14C]triolein intragastrically, while others received an intraperitoneal injection of 3 mCi 3H2O. All animals were killed by decapitation. the retroperitoneal, visceral epididymal and omental white adipose tissues, brown adipose tissue, liver and blood were immediately removed. the lipid uptake from the diet, in vivo rate of lipogenesis, percentage of fat, lipid profile and leptin concentration were analysed. the high-fat diet promoted an increase in fatty liver (P= 0.05), adiposity mass (P= 0.05) and the plasma concentration of leptin (P= 0.05) and a decreased lipid uptake in white adipose tissue depots (P= 0.05) in relation to the chow diet. the moderate caloric restriction did not reverse the changes promoted by the high-fat diet but induced a small decrease in adiposity, which was reversed after refeeding, and the animals maintained a dyslipidaemic profile and high fat deposition in the liver. We can conclude that the high-fat diet and subsequent moderate caloric restriction plus refeeding increased the risks of developing visceral obesity, dyslipidaemia and non-alcoholic fatty liver disease, which suggests that this type of experimental protocol can be used to study mechanisms related to the metabolic syndrome.en
dc.description.affiliationUniv Fed Sao Carlos, Dept Educ Fis & Motricidade Humana, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Ciencias Fisiol, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Fed Uberlandia, Fac Educ Fis & Fisioterapia, BR-38400 Uberlandia, MG, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Programa Posgrad Biotecnol, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biociencias, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Programa Posgrad Interdisciplinar Ciencias Saude, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biociencias, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Programa Posgrad Interdisciplinar Ciencias Saude, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCNPq: 141486/2003-3
dc.description.sponsorshipIDFAPESP: 99/12981-7
dc.format.extent882-894
dc.identifierhttps://dx.doi.org/10.1113/expphysiol.2011.064121
dc.identifier.citationExperimental Physiology. Hoboken: Wiley-Blackwell, v. 97, n. 7, p. 882-894, 2012.
dc.identifier.doi10.1113/expphysiol.2011.064121
dc.identifier.issn0958-0670
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/35019
dc.identifier.wosWOS:000306281700007
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofExperimental Physiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.titleFood restriction and refeeding induces changes in lipid pathways and fat deposition in the adipose and hepatic tissues in rats with diet-induced obesityen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ISS - Artigos