Voltage-Gated Proton Channel in Human Glioblastoma Multiforme Cells

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dc.citation.issue7
dc.citation.volume7
dc.contributor.authorRibeiro-Silva, Luisa [UNIFESP]
dc.contributor.authorQueiroz, Fernanda Oliveira [UNIFESP]
dc.contributor.authorBrito da Silva, Annielle Mendes [UNIFESP]
dc.contributor.authorHirata, Aparecida Emiko [UNIFESP]
dc.contributor.authorArcisio-Miranda, Manoel [UNIFESP]
dc.coverageWashington
dc.date.accessioned2020-08-14T13:44:25Z
dc.date.available2020-08-14T13:44:25Z
dc.date.issued2016
dc.description.abstractSolid tumors tend to have a more glycolytic metabolism leading to an accumulation of acidic metabolites in their cytosol, and consequently, their intracellular pH (pH(i)) turns critically lower if the cells do not handle the acid excess. Recently, it was proposed that the voltage gated proton channels (H(v)1) can regulate the pH(i) in several cancers. Here we report the functional expression of voltage gated proton channels in a human glioblastoma multiforme (GBM) cell line, the most common and lethal brain tumor. T98G cells presented an outward, slow activating voltage-dependent proton current, which was also Delta pH-dependent and inhibited by ZnCl2, characterizing it as being conducted by H(v)1 channels. Furthermore, blocking H(v)1 channels with ZnCl2 significantly reduced the pH(i), cell survival, and migration, indicating an important role for H(v)1 for tumor proliferation and progression in GBM. Overall, our results suggest that H(v)1 channels can be a new therapeutic target for GBM.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Lab Neurobiol Estrutural & Func LaNEF, BR-04023060 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Fisiol, BR-04023060 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Lab Neurobiol Estrutural & Func LaNEF, BR-04023060 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Fisiol, BR-04023060 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (Processo Fapesp)
dc.description.sponsorshipFapesp fellowship
dc.description.sponsorshipCapes fellowship
dc.description.sponsorshipIDFAPESP: 2010/52077-9
dc.description.sponsorshipIDFAPESP: 2011/52099-5
dc.description.sponsorshipIDFAPESP: 2013/26005-9
dc.format.extent864-869
dc.identifierhttp://dx.doi.org/10.1021/acschemneuro.6b00083
dc.identifier.citationAcs Chemical Neuroscience. Washington, v. 7, n. 7, p. 864-869, 2016.
dc.identifier.doi10.1021/acschemneuro.6b00083
dc.identifier.issn1948-7193
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57656
dc.identifier.wosWOS:000380297500004
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Chemical Neuroscience
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectProton channelen
dc.subjectintracellular pHen
dc.subjectgliomaen
dc.titleVoltage-Gated Proton Channel in Human Glioblastoma Multiforme Cellsen
dc.typeinfo:eu-repo/semantics/article
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