Characterization of a novel cystatin type 2 from Rhipicephalus microplus midgut

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dc.contributor.authorCardoso, Thyago H. S. [UNIFESP]
dc.contributor.authorLu, Stephen [UNIFESP]
dc.contributor.authorGonzalez, Boris R. G. [UNIFESP]
dc.contributor.authorTorquato, Ricardo J. S. [UNIFESP]
dc.contributor.authorTanaka, Aparecida S. [UNIFESP]
dc.date.accessioned2019-08-19T11:48:38Z
dc.date.available2019-08-19T11:48:38Z
dc.date.issued2017
dc.description.abstractThe Rhipicephalus (Boophilus) microplus is an exclusive bovine ectoparasite responsible for the transmission of pathogens that decrease meat, leather and milk productions. Cattle vaccination is an alternative to control tick infestations, but the discovery of potential antigens is still a challenge for researchers. Recently, our group performed a midgut transcriptome of engorged R. microplus tick, and out of 800-ESTs sequences one cystatin-coding sequence was identified and named Rmcystatin-4. In order to understand the physiological role of Rmcystatin-4, the aim of this work was the expression, purification and functional characterization of a novel type 2 cystatin from the tick R. microplus. Rmcystatin-4 gene expression was identified mostly in tick midgut suggesting its possible role in blood digestion control. Our data showed that rRmcystatin-4 was successfully expressed in active form using Pichia pastoris system and the purified inhibitor presented high selectivity to BmCl-1 (Ki = 0.046 nM). Moreover, rRmcystatin-4 was able to impaired BmCl-1 activity towards bovine hemoglobin. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.en
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Escola Paulista Med, Dept Biochem, Rua 3 Maio 100, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, UNIFESP, Escola Paulista Med, Dept Biochem, Rua 3 Maio 100, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipINCT-Entomologia Molecular (CNPq)
dc.description.sponsorshipIDFAPESP: 2012/03657-8
dc.description.sponsorshipIDFAPESP: 2011/09340-3
dc.description.sponsorshipIDCNPq: 308780/2013-2
dc.description.sponsorshipIDCNPq: 313073/2013-9
dc.description.sponsorshipIDINCT: 465678/2014-9
dc.format.extent117-121
dc.identifierhttp://dx.doi.org/10.1016/j.biochi.2017.07.005
dc.identifier.citationBiochimie. Paris, v. 140, p. 117-121, 2017.
dc.identifier.doi10.1016/j.biochi.2017.07.005
dc.identifier.issn0300-9084
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51307
dc.identifier.wosWOS:000410870000012
dc.language.isoeng
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCystatinen
dc.subjectCysteine proteasesen
dc.subjectTicken
dc.subjectMidguten
dc.titleCharacterization of a novel cystatin type 2 from Rhipicephalus microplus midguten
dc.typeinfo:eu-repo/semantics/article
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