A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults

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dc.contributor.authorPaniz, Clovis
dc.contributor.authorBertinato, Juliano Felix
dc.contributor.authorLucena, Maylla Rodrigues [UNIFESP]
dc.contributor.authorDe Carli, Eduardo
dc.contributor.authorda Silva Amorim, Patricia Mendonca
dc.contributor.authorGomes, Guilherme Wataru
dc.contributor.authorPalchetti, Cecilia Zanin
dc.contributor.authorFigueiredo, Maria Stella [UNIFESP]
dc.contributor.authorPfeiffer, Christine M.
dc.contributor.authorFazili, Zia
dc.contributor.authorGreen, Ralph
dc.contributor.authorGuerra-Shinohara, Elvira Maria [UNIFESP]
dc.date.accessioned2019-08-19T11:48:31Z
dc.date.available2019-08-19T11:48:31Z
dc.date.issued2017
dc.description.abstractBackground: The effects of high-dose folic acid (FA) supplementation in healthy individuals on blood folate concentrations and immune response are unknown. Objective: The aim of the study was to evaluate the effects of daily consumption of a tablet containing 5 mg FA on serum folateen
dc.description.abstractnumber and cytotoxicity of natural killer (NK) cellsen
dc.description.abstractmRNA expression of dihydrofolate reductase (DHFR), methylenetetrahydrofolate reductase (MTHFR), interferon gamma (IFNG), tumor necrosis factor a (TNFA), and interleukin 8 (IL8) genesen
dc.description.abstractand concentrations of serum inflammatory markers. Methods: This prospective clinical trial was conducted in 30 healthy Brazilian adults (15 women), aged 27.7 y (95% CI: 26.4, 29.1 y), with a body mass index (in kg/m 2) of 23.1 (95% CI: 22.0, 24.3). Blood was collected at baseline and after 45 and 90 d of the intervention. Serum folate concentrations were measured by microbiological assay and HPLC-tandem mass spectrometry [ folate forms, including unmetabolized folic acid (UMFA)]. We used real-time polymerase chain reaction to assess mononuclear leukocyte mRNA expression and flow cytometry to measure the number and cytotoxicity of NK cells. Results: Serum folate concentrations increased byen
dc.description.abstract5-fold after the intervention (P < 0.001), and UMFA concentrations increased by 11.9-and 5.9-fold at 45 and 90 d, respectively, when compared with baseline (P < 0.001). UMFA concentrations increased (> 1.12 nmol/L) in 29 (96.6%) participants at day 45 and in 26 (86.7%) participants at day 90. We observed significant reductions in the number (P < 0.001) and cytotoxicity (P = 0.003) of NK cells after 45 and 90 d. Compared with baseline, DHFR mRNA expression was higher at 90 d (P = 0.006) and IL8 and TNFA mRNA expressions were higher at 45 and 90 d (P = 0.001 for both). Conclusion: This noncontrolled intervention showed that healthy adults responded to a high-dose FA supplement with increased UMFA concentrations, changes in cytokine mRNA expression, and reduced number and cytotoxicity of NK cells.en
dc.description.affiliationUniv São Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Food & Expt Nutr, Fac Pharmaceut Sci, São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Hematol & Blood Transfus Div, São Paulo, Brazil
dc.description.affiliationCDC, Natl Ctr Environm Hlth, Atlanta, GA 30333 USA
dc.description.affiliationUC Davis Sch Med, Dept Pathol & Lab Med, Sacramento, CA USA
dc.description.affiliationUnifespUniv Fed São Paulo, Hematol & Blood Transfus Div, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2012/12912-1
dc.description.sponsorshipIDCNPq: 482641/2012-6
dc.description.sponsorshipIDCNPq: 401586/2014-6
dc.format.extent1684-1692
dc.identifierhttp://dx.doi.org/10.3945/jn.117.247445
dc.identifier.citationJournal Of Nutrition. Bethesda, v. 147, n. 9, p. 1684-1692, 2017.
dc.identifier.doi10.3945/jn.117.247445
dc.identifier.issn0022-3166
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51271
dc.identifier.wosWOS:000411807800012
dc.language.isoeng
dc.publisherAmer Soc Nutrition-Asn
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectfolic acid supplementationen
dc.subjectunmetabolized folic aciden
dc.subjectNK cell cytotoxicityen
dc.subjectmRNA expressionen
dc.subjectserum folateen
dc.subject5-methyl-THFen
dc.titleA Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adultsen
dc.typeinfo:eu-repo/semantics/article
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