Morphometric changes in C57BL/6 mice retina infected by Toxoplasma gondii ME 49 strain

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dc.contributor.authorHigino Rocha, Ana Cristina
dc.contributor.authorCalabrese, Katia da Silva
dc.contributor.authorTedesco, Roberto Carlos [UNIFESP]
dc.contributor.authorCampos, Wesley Ribeiro
dc.contributor.authorNeto, Miguel Houri
dc.contributor.authorVasconcelos, Anilton Cezar
dc.contributor.authorOrefice, Fernando
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:03Z
dc.date.available2016-01-24T14:35:03Z
dc.date.issued2014-01-01
dc.description.abstractThis study evaluated the morphometric implications in C57BL/6 mouse retina infected by Toxoplasma gondii, ME 49 strain. Twenty C57BL/6 female mice were divided into group 1 (n = 8, intraperitoneally infected with 30 cysts of T. gondii ME 49 strain) and group 2 (n = 12 non-infected controls). the eyes were enucleated on the 60th day after infection, fixed and processed for light microscopy. Changes in retinal thickness and in the perimeter/area ratio (P/A) of the retinal layers were analyzed by digital morphometry. We considered that P/A was the measurement of retinal architecture distortion induced by toxoplasmosis. This study considered the ganglion cells and nerve fiber layers as a monolayer, thus six layers of retina were evaluated: photoreceptors (PRL), outer nuclear (ONL), outer plexiform (OPL), inner nuclear (INL), inner plexiform (IPL) and ganglion cells/nerve fiber monolayer (GNL). Histological analysis of infected mouse retina showed inflammatory infiltrate, necrosis, glial reaction and distortion of the retina architecture. It also presented increased thickness (167.8 +/- 24.9 mu m versus 121.1 +/- 15.4 mu m, in controls) and increased retinal thickness within the retinitis foci (187.7 +/- 16.6 mu m versus 147.9 +/- 12.2 mu m out of the retinitis foci). A statistically significant difference in P/A was observed between infected and uninfected mouse retinas. the same was observed in PRL, ON, INL and GNL. Retinal morphometry may be used to demonstrate differences between infected and uninfected mouse retinas. Published by Elsevier Inc.en
dc.description.affiliationUniv Fed Minas Gerais, Hosp Clin, Hosp Sao Geraldo, Fac Med, Belo Horizonte, MG, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Imunomodulacao & Protozool, Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Anat Descrit & Topog, São Paulo, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Fac Med, Dept Oftalmol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, ICB, Dept Patol, Lab Apoptose, Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Anat Descrit & Topog, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1-4
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2013.10.013
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 136, p. 1-4, 2014.
dc.identifier.doi10.1016/j.exppara.2013.10.013
dc.identifier.issn0014-4894
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37239
dc.identifier.wosWOS:000329952100001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMiceen
dc.subjectOcular toxoplasmosisen
dc.subjectMorphometryen
dc.subjectEyeen
dc.titleMorphometric changes in C57BL/6 mice retina infected by Toxoplasma gondii ME 49 strainen
dc.typeinfo:eu-repo/semantics/article
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