Susceptibility of Sporothrix brasiliensis isolates to amphotericin B, azoles, and terbinafine

Página do item simplificado
dc.contributor.authorBorba-Santos, Luana Pereira
dc.contributor.authorRodrigues, Anderson Messias [UNIFESP]
dc.contributor.authorGagini, Thalita Braga
dc.contributor.authorFernandes, Geisa Ferreira [UNIFESP]
dc.contributor.authorCastro, Rafaela
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.authorNucci, Marcio
dc.contributor.authorLopes-Bezerra, Leila Maria
dc.contributor.authorIshida, Kelly
dc.contributor.authorRozental, Sonia
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:40:01Z
dc.date.available2016-01-24T14:40:01Z
dc.date.issued2015-02-01
dc.description.abstractThe in vitro activity of the antifungal agents amphotericin B (AMB), itraconazole (ITC), posaconazole (PSC), voriconazole (VRC), and terbinafine (TRB) against 32 Brazilian isolates of Sporothrix brasiliensis, including 16 isolates from a recent (2011-2012) epidemic in Rio de Janeiro state, was examined. We describe and genotype new isolates and clustered them with 16 older (from 2004 or earlier) S. brasiliensis isolates by phylogenetic analysis. We tested both the yeast and the mycelium form of all isolates using broth microdilution methods based on the reference protocols M38-A2 and M27-A3 (recommended by the Clinical and Laboratory Standards Institute). Considering minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs), TRB was found to be the most active drug in vitro for both fungal forms, followed by PSC. Several isolates showed high MICs for AMB and/or ITC, which are currently used as first-line therapy for sporotrichosis. VRC displayed very low activity against S. brasiliensis isolates. the primary morphological modification observed on treated yeasts by transmission electron microscopy analysis was changes in cell wall. Our results indicate that TRB is the antifungal with the best in vitro activity against S. brasiliensis and support the use of TRB as a promising option for the treatment of cutaneous and/or lymphocutaneous sporotrichosis.en
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Biophys Carlos Chagas Filho, BR-21941902 Rio de Janeiro, RJ, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Estado Rio de Janeiro, Dept Cell Biol, Rio de Janeiro, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Univ Hosp Clementino Fraga Filho, BR-21941902 Rio de Janeiro, RJ, Brazil
dc.description.affiliationUniv São Paulo, Dept Microbiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento e Pesquisa
dc.format.extent178-188
dc.identifierhttp://dx.doi.org/10.1093/mmy/myu056
dc.identifier.citationMedical Mycology. Oxford: Oxford Univ Press, v. 53, n. 2, p. 178-188, 2015.
dc.identifier.doi10.1093/mmy/myu056
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38720
dc.identifier.wosWOS:000349745700012
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofMedical Mycology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectSporothrix brasiliensisen
dc.subjectantifungal susceptibilityen
dc.subjectamphotericin Ben
dc.subjectazolesen
dc.subjectterbinafineen
dc.subjectultrastructureen
dc.titleSusceptibility of Sporothrix brasiliensis isolates to amphotericin B, azoles, and terbinafineen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral