Inflammation-induced modulation of cellular galectin-1 and-3 expression in a model of rat peritonitis

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dc.contributor.authorGil, C. D.
dc.contributor.authorCooper, D.
dc.contributor.authorRosignoli, G.
dc.contributor.authorPerretti, M.
dc.contributor.authorOliani, S. M.
dc.contributor.institutionUNESP
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionQueen Mary Sch Med & Dent
dc.date.accessioned2016-01-24T12:41:00Z
dc.date.available2016-01-24T12:41:00Z
dc.date.issued2006-03-01
dc.description.abstractObjective and design: To investigate the effect of galectin-1 (Gal-1) and -3 (Gal-3) on leukocyte migration and analyze the expression of both galectins in inflammatory cells using a model of rat peritonitis.Material or Subjects: Sprague-Dawley rats (n = 4 per group).Treatment: Peritonitis was induced in animals through intraperitoneal injection of carrageenin (1.5 mg/kg) and rat mesenteries were analyzed at different time points (0, 4, 24 and 48h). for pharmacological treatment, rats received intravenous injection of Gal-1 or -3 (3 mu g/kg) followed by carrageenin.Methods: Western blotting and immunoelectron microscopy analysis. Statistical analysis was performed using ANOVA followed by Bonferroni test.Results: Pharmacological treatment with Gal-1, but not Gal-3, inhibited (similar to 50%) leukocyte recruitment into the peritoneal cavity at 4h time-point. in this early phase, immunogold staining of mesenteries showed a diminished Gal-3 expression in degranulated mast cells and Gal-1 in transmigrated neutrophils (similar to 20% reduction compared to intravascular cells). in the later phases (24 and 48 h), leukocyte turnover was associated with augmented Gal-1 expression in neutrophils and macrophages and Gal-3 in mast cells and macrophages.Conclusions: These results point to a balanced expression of cell-associated-Gal-1/Gal-3 and might impact on the development of new therapeutic strategies for inflammatory diseases.en
dc.description.affiliationUNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationFAMERP, Fac Med, Dept Agron, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUNIFESP, São Paulo Sch Med, Postgrad Morphol, BR-04023900 São Paulo, Brazil
dc.description.affiliationQueen Mary Sch Med & Dent, William Harvey Res Inst, London ECIM 6BQ, England
dc.description.affiliationUnifespUNIFESP, São Paulo Sch Med, Postgrad Morphol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent99-107
dc.identifierhttp://dx.doi.org/10.1007/s00011-005-0059-4
dc.identifier.citationInflammation Research. Basel: Birkhauser Verlag Ag, v. 55, n. 3, p. 99-107, 2006.
dc.identifier.doi10.1007/s00011-005-0059-4
dc.identifier.issn1023-3830
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28764
dc.identifier.wosWOS:000236559700003
dc.language.isoeng
dc.publisherBirkhauser Verlag Ag
dc.relation.ispartofInflammation Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectIn vivo inflammationen
dc.subjectmast cellen
dc.subjectneutrophilen
dc.subjectendothelial cellen
dc.subjectmacrophageen
dc.titleInflammation-induced modulation of cellular galectin-1 and-3 expression in a model of rat peritonitisen
dc.typeinfo:eu-repo/semantics/article
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