N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

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dc.contributor.authorAmorim, Elivânia Marques Gomes de
dc.contributor.authorDamous, Luciana Lamarão [UNIFESP]
dc.contributor.authorDurando, Maria Clara Silva
dc.contributor.authorSaraiva, Márcia Viviane Alves
dc.contributor.authorKoike, Marcia Kiyomi
dc.contributor.authorMontero, Edna Frasson de Souza [UNIFESP]
dc.contributor.institutionFederal University do Vale do São Francisco
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversity of São Paulo Medical School Obstetrics and Gynecology Department
dc.contributor.institutionFederal University of Ceara Faculty of Medicine
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-06-14T13:46:48Z
dc.date.available2015-06-14T13:46:48Z
dc.date.issued2014-01-01
dc.description.abstractPURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC.METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test.RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation.CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.en
dc.description.affiliationFederal University do Vale do São Francisco
dc.description.affiliationUNIFESP
dc.description.affiliationUniversity of São Paulo Medical School Obstetrics and Gynecology Department
dc.description.affiliationFederal University of Ceara Faculty of Medicine
dc.description.affiliationUSP FM Department of Clinical Medicine
dc.description.affiliationUnifespUNIFESP
dc.description.sourceSciELO
dc.format.extent22-27
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001700005
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 22-27, 2014.
dc.identifier.doi10.1590/S0102-86502014001700005
dc.identifier.fileS0102-86502014001500022.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001500022
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8125
dc.identifier.wosWOS:000344466300006
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsAcesso aberto
dc.subjectTransplantationen
dc.subjectAutologousen
dc.subjectAcetylcysteineen
dc.subjectHistologyen
dc.subjectEstrogenen
dc.subjectOvariumen
dc.subjectRatsen
dc.titleN-acetylcysteine improves morphologic and functional aspects of ovarian grafts in ratsen
dc.typeArtigo
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