A reversible thermoresponsive sealant for temporary closure of ocular trauma

Página do item simplificado
dc.citation.issue419
dc.citation.volume9
dc.contributor.authorBayat, Niki
dc.contributor.authorZhang, Yi
dc.contributor.authorFalabella, Paulo [UNIFESP]
dc.contributor.authorMenefee, Roby
dc.contributor.authorWhalen, John J., III
dc.contributor.authorHumayun, Mark S.
dc.contributor.authorThompson, Mark E.
dc.coverageWashington
dc.date.accessioned2020-07-02T18:52:20Z
dc.date.available2020-07-02T18:52:20Z
dc.date.issued2017
dc.description.abstractOpen globe injuries are full-thickness injuries sustained to the eye wall (cornea or sclera), which cause immediate drops in intraocular pressure that may lead to retinal detachment and permanent vision loss if not treated rapidly after injury. The current standard of care for open globe injuries consists of suturing the margins closed, but the technique can be time-consuming, requires specialized training and equipment, and can lead to patient discomfort, abrasion, and infection from eye rubbing. We engineered an injectable, thermoresponsive sealant (TRS) and a custom tool to occlude open globe injuries. The smart hydrogel sealant consists of physically cross-linked N-iso-propylacrylamide copolymerized with butylacrylate. At low temperatures, it can be injected as a liquid, and when raised to body temperature, a heat-induced gelation converts the hydrogel into a solidified occlusion. The sealant can be repositioned or removed without causing additional trauma via exposure to cold water. In vitro and ex vivo assessments of mechanical adhesion to eye tissue revealed maintenance of intraocular pressure that is five times greater than the physiological range with reversible seal strength comparable to cyanoacrylate (super glue). In vivo assessment in a rabbit model of ocular trauma demonstrated ease of use for TRS deployment, statistically significant improvement in wound sealing, and no evidence of neurotoxicity, retinal tissue degradation, or significant chronic inflammatory response after 30 days of exposure. Given the advantages of body heat-induced gelation, rapid reversible occlusion, and in vivo safety and efficacy, shape-adaptable TRSs have translational potential as smart wound sealants for temporary occlusion of surgical incisions or traumatic injuries.en
dc.description.affiliationUniv Southern Calif, Mork Family Dept Chem Engn & Mat Sci, Los Angeles, CA 90089 USA
dc.description.affiliationUniv Southern Calif, USC Roski Eye Inst, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA 90033 USA
dc.description.affiliationUniv Southern Calif, USC Inst Biomed Therapeut, Keck Sch Med, Los Angeles, CA 90033 USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Ophthalmol & Visual Sci, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Southern Calif, Dept Biomed Engn, Denney Res Ctr 140, 1042 Downey Way, Los Angeles, CA 90089 USA
dc.description.affiliationUniv Southern Calif, Dept Neurosci, 3641 Watt Way,HNB 120, Los Angeles, CA 90089 USA
dc.description.affiliationUniv Southern Calif, Dept Chem, Los Angeles, CA 90089 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ophthalmol & Visual Sci, BR-04023062 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipOffice of the Assistant Secretary of Defense for Health Affairs through FY12 Vision Research Program [W81XWH12-1-0314]
dc.description.sponsorshipNIH through SC-CTSI program [NIH/National Center for Research Resources (NCRR)/National Center for Advancing Translational Science (NCATS)] from NCATS [UL1TR000130, UL1TR001855]
dc.description.sponsorshipNSF [EEC-0310723]
dc.description.sponsorshipResearch to Prevent Blindness
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1126/scitranslmed.aan3879
dc.identifier.citationScience Translational Medicine. Washington, v. 9, n. 419, p. -, 2017.
dc.identifier.doi10.1126/scitranslmed.aan3879
dc.identifier.fileWOS000417196900003.pdf
dc.identifier.issn1946-6234
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54007
dc.identifier.wosWOS:000417196900003
dc.language.isoeng
dc.publisherAmer Assoc Advancement Science
dc.relation.ispartofScience Translational Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleA reversible thermoresponsive sealant for temporary closure of ocular traumaen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000417196900003.pdf
Tamanho:
7.65 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos