Diagnosis of Helicobacter pylori Infection by Means of Reduced-Dose C-13-Urea Breath Test and Early Sampling of Exhaled Breath

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dc.contributor.authorPacheco, Sylmara L. M. [UNIFESP]
dc.contributor.authorOgata, Silvio Kazuo [UNIFESP]
dc.contributor.authorMachado, Rodrigo S. [UNIFESP]
dc.contributor.authorSilva Patricio, Francy Reis da [UNIFESP]
dc.contributor.authorPardo, Mario L. E. [UNIFESP]
dc.contributor.authorKawakami, Elisabete [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:34:37Z
dc.date.available2016-01-24T14:34:37Z
dc.date.issued2013-11-01
dc.description.abstractObjective:The aim of this study was to evaluate the accuracy of reduced-dose C-13-urea breath test (C-13-UBT) and early sampling of exhaled breath for the detection of Helicobacter pylori infection in children and adolescents.Methods:Patients up to 20 years old that underwent upper gastrointestinal endoscopy with gastric biopsies were included. the C-13-UBT was performed after a 4-hour fasting period with 4 points of collection: baseline (T-0), and at 10, 20, and 30 minutes (T-10, T-20, and T-30) after ingestion of 25 mg C-13-urea diluted in 100 mL of apple juice. the infection status was defined through 3 invasive methods, and a patient was considered infected with a positive culture or concomitant positive histology and rapid urease test. the absence of infection was defined by all negative histology, rapid urease test, and culture. Analysis of exhaled breath samples was performed with an isotope-selective infrared spectrometer. A receiver-operating characteristic curve analysis was done to define cutoff delta over baseline (DOB) values.Results:A total of 129 patients between the ages of 2.1 and 19 years (median 11.6 years; mean agestandard deviation 11.5 +/- 3.8 years; F:M 85:44) were included. the prevalence of infection was 41.1%. the sensitivity (S) and specificity (Sp) were at T-10 (cutoff DOB 2.55 parts per thousand), S 94.7% (95% confidence interval [CI] 90.9-98.5) and Sp 96.8% (95% CI 93.4-100); at T-20 (DOB 2.5 parts per thousand), S 96.2% (95% CI 92.9-99.5) and Sp 96.1% (95% CI 93.7-99.8); and at T-30 (DOB 1.6 parts per thousand), S 96.2% (95% CI 92.9-99.5) and Sp 94.7% (95% CI 90.8-98.6).Conclusions:Low-dose C-13-UBT with early sampling is accurate for diagnosing H pylori infection in children and adolescents.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med UNIFESP EPM, Dept Pediat, Pediat Gastroenterol Div, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med UNIFESP EPM, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med UNIFESP EPM, Dept Pediat, Pediat Gastroenterol Div, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med UNIFESP EPM, Dept Pathol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent607-611
dc.identifierhttp://dx.doi.org/10.1097/MPG.0b013e3182a02608
dc.identifier.citationJournal of Pediatric Gastroenterology and Nutrition. Philadelphia: Lippincott Williams & Wilkins, v. 57, n. 5, p. 607-611, 2013.
dc.identifier.doi10.1097/MPG.0b013e3182a02608
dc.identifier.issn0277-2116
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36900
dc.identifier.wosWOS:000326745900016
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofJournal of Pediatric Gastroenterology and Nutrition
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectC-13-urea breath testen
dc.subjectHelicobacter infectionen
dc.subjectdiagnosisen
dc.subjecttime factorsen
dc.subjectureaen
dc.subjectadministration and dosageen
dc.titleDiagnosis of Helicobacter pylori Infection by Means of Reduced-Dose C-13-Urea Breath Test and Early Sampling of Exhaled Breathen
dc.typeinfo:eu-repo/semantics/article
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