Rats With Different Thresholds to Clonic Convulsions Induced by DMCM Differ in the Binding of [H-3]-MK-801 and [H-3]-Ouabain in the Membranes of Brain Regions
| dc.contributor.author | Contó, Marcos Brandão [UNIFESP] | |
| dc.contributor.author | Barbosa de Carvalho, Jose Gilberto [UNIFESP] | |
| dc.contributor.author | Venditti, Marco Antonio Campana [UNIFESP] | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T14:27:25Z | |
| dc.date.available | 2016-01-24T14:27:25Z | |
| dc.date.issued | 2012-07-01 | |
| dc.description.abstract | Considering the putative participation of N-methyl-D-aspartate (NMDA) receptors and the Na+, K+-ATPase enzymes in the susceptibility to convulsions induced by the benzodiazepine inverse agonist methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate (DMCM), the present study sought to determine if rats with high (HTR) and low (LTR) thresholds to clonic convulsions induced by DMCM differed in the following aspects: the binding of NMDA receptors by [H-3]-MK-801, Na+, K+-ATPase activity (K+-stimulated p-nitrophenylphosphatase) and high-affinity [H-3]-ouabain binding to membranes from discrete brain regions. Compared to the HTR subgroup, the LTR subgroup presented a lower binding of [H-3]-MK-801 in the hippocampus, frontal cortex and striatum. the subgroups did not differ in K+-p-nitrophenylphosphatase activity, but the LTR subgroup had a lower density of isozymes with a high-affinity to ouabain in the brainstem and in the frontal cortex and a lower affinity to ouabain in the hippocampus than the HTR subgroup. These results suggest that NMDA receptors and ouabain-sensitive Na+, K+-ATPase isozymes may underlie the susceptibility to DMCM-induced convulsions. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Dept Psicobiol, Escola Paulista Med UNIFESP EPM, BR-04023062 São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Dept Psicobiol, Escola Paulista Med UNIFESP EPM, BR-04023062 São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.description.sponsorship | Associacao Fundo de Incentivo a Pesquisa (AFIP) | |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.format.extent | 1442-1449 | |
| dc.identifier | http://dx.doi.org/10.1007/s11064-012-0730-4 | |
| dc.identifier.citation | Neurochemical Research. New York: Springer/plenum Publishers, v. 37, n. 7, p. 1442-1449, 2012. | |
| dc.identifier.doi | 10.1007/s11064-012-0730-4 | |
| dc.identifier.issn | 0364-3190 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/35047 | |
| dc.identifier.wos | WOS:000304878200008 | |
| dc.language.iso | eng | |
| dc.publisher | Springer | |
| dc.relation.ispartof | Neurochemical Research | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
| dc.subject | [3H]-ouabain | en |
| dc.subject | [3H]-MK 801 | en |
| dc.subject | Na plus , K plus -ATPase | en |
| dc.subject | Convulsion | en |
| dc.subject | DMCM | en |
| dc.title | Rats With Different Thresholds to Clonic Convulsions Induced by DMCM Differ in the Binding of [H-3]-MK-801 and [H-3]-Ouabain in the Membranes of Brain Regions | en |
| dc.type | info:eu-repo/semantics/article |