Effect of hydroalcoholic extract from Copaifera langsdorffii leaves on urolithiasis induced in rats

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dc.contributor.authorBrancalion, Ana Paula S.
dc.contributor.authorOliveira, Rejane B.
dc.contributor.authorSousa, Joao Paulo B.
dc.contributor.authorGroppo, Milton
dc.contributor.authorBerretta, Andresa A.
dc.contributor.authorBarros, Marcio E. [UNIFESP]
dc.contributor.authorBoim, Mirian A. [UNIFESP]
dc.contributor.authorBastos, Jairo K.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionApis Flora Ind & Comercial Ltda
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:47Z
dc.date.available2016-01-24T14:27:47Z
dc.date.issued2012-10-01
dc.description.abstractCopaifera langsdorffii Desf. commonly known as copaiba, produce a commercially valuable oil-resin that is extensively used in folk medicine for anti-inflammatory, antimicrobial and antiseptic purposes. We have found the hydroalcoholic extract of this plant leaf has the potential to treat urolithiasis, a problem affecting similar to 7% of the population. To isolate the functional compounds C. langsdorffii leaves were dried, ground, and macerated in a hydroalcoholic solution 7:3 to produce a 16.8% crude extract after solvent elimination. Urolithiasis was induced by introduction of a calcium oxalate pellet (CaOx) into the bladders of adult male Wistar rats. the treated groups received the crude extract by oral gavage at 20 mg/kg body weight daily for 18 days. Extract treatment started 30 days after CaOx seed implantation. To monitor renal function sodium, potassium and creatinine concentrations were analyzed in urine and plasma, and were found to be in the normal range. Analyses of pH, magnesium, phosphate, calcium, uric acid, oxalate and citrate levels were evaluated to determine whether the C. langsdorffii extract may function as a stone formation prevention agent. the HPLC analysis of the extract identified flavonoids quercitrin and afzelin as the major components. Animals treated with C. langsdorffii have increased levels of magnesium and decreased levels of uric acid in urinary excretions. Treated animals have a significant decrease in the mean number of calculi and a reduction in calculi mass. Calculi taken from extract treated animals were more brittle and fragile than calculi from untreated animals. Moreover, breaking calculi from untreated animals required twice the amount of pressure as calculi from treated animals (6.90 +/- A 3.45 vs. 3.00 +/- A 1.51). the extract is rich in flavonoid heterosides and other phenolic compounds. Therefore, we hypothesize this class of compounds might contribute significantly to the observed activity.en
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationApis Flora Ind & Comercial Ltda, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2008/57775-5
dc.format.extent475-481
dc.identifierhttp://dx.doi.org/10.1007/s00240-011-0453-z
dc.identifier.citationUrological Research. New York: Springer, v. 40, n. 5, p. 475-481, 2012.
dc.identifier.doi10.1007/s00240-011-0453-z
dc.identifier.issn0300-5623
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35340
dc.identifier.wosWOS:000308813800006
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofUrological Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectCopaifera langsdorffiien
dc.subjectUrolithiasisen
dc.subjectPhenolic compoundsen
dc.subjectFlavonoid heterosidesen
dc.titleEffect of hydroalcoholic extract from Copaifera langsdorffii leaves on urolithiasis induced in ratsen
dc.typeinfo:eu-repo/semantics/article
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