Developing skeletal muscle cells express functional muscarinic acetylcholine receptors coupled to different intracellular signaling systems
| dc.contributor.author | Furlan, Ingrid | |
| dc.contributor.author | Godinho, Rosely Oliveira [UNIFESP] | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T12:38:04Z | |
| dc.date.available | 2016-01-24T12:38:04Z | |
| dc.date.issued | 2005-10-01 | |
| dc.description.abstract | 1 This study analyzed the expression of muscarinic acetylcholine receptors (mAChRs) in the rat cultured skeletal muscle cells and their coupling to G protein, phospholipase C and adenylyl cyclase (AC).2 Our results showed the presence of a homogeneous population of [H-3]methyl-quinuclidinyl benzilate-binding sites in the membrane fraction from the rat cultured muscle (K-D = 0.4 nM, B-max = 8.9 fmol mg protein(-1)). Specific muscarinic binding sites were also detected in denervated diaphragm muscles from adult rats and in myoblasts isolated from newborn rats.3 Activation of mAChRs with carbachol induced specific [S-35]GTP gamma S binding to cultured muscle membranes and potentiated the forskolin-dependent stimulation of AC. These effects were totally inhibited by 0.1-1 mu M atropine.4 in addition, mAChRs were able to stimulate generation of diacylglycerol (DAG) in response to acetylcholine, carbachol or selective mAChR agonist oxotremorine-M.5 the carbachol-dependent increase in DAG was inhibited in a concentration-dependent manner by mAChR antagonists atropine, pirenzepine and 4-DAMP mustard.6 Finally, activation of these receptors was correlated with increased synthesis of acetylcholinesterase, via a PKC-dependent pathway.7 Taken together, these results indicate that expression of mAChRs, coupled to G protein and distinct intracellular signaling systems, is a characteristic of noninnervated skeletal muscle cells and may be responsible for trophic influences of acetylcholine during formation of the neuromuscular synapse. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Dept Pharmacol, Div Cellular Pharmacol, Escola Paulista Med, BR-04044020 São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Dept Pharmacol, Div Cellular Pharmacol, Escola Paulista Med, BR-04044020 São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.format.extent | 389-396 | |
| dc.identifier | http://dx.doi.org/10.1038/sj.bjp.0706329 | |
| dc.identifier.citation | British Journal of Pharmacology. London: Nature Publishing Group, v. 146, n. 3, p. 389-396, 2005. | |
| dc.identifier.doi | 10.1038/sj.bjp.0706329 | |
| dc.identifier.issn | 0007-1188 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/28475 | |
| dc.identifier.wos | WOS:000232317400010 | |
| dc.language.iso | eng | |
| dc.publisher | Nature Publishing Group | |
| dc.relation.ispartof | British Journal of Pharmacology | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | muscarinic acetylcholine receptors | en |
| dc.subject | diacylglycerol | en |
| dc.subject | cyclic AMP | en |
| dc.subject | skeletal muscle | en |
| dc.subject | G protein | en |
| dc.subject | development | en |
| dc.title | Developing skeletal muscle cells express functional muscarinic acetylcholine receptors coupled to different intracellular signaling systems | en |
| dc.type | info:eu-repo/semantics/article |