Calcium phosphate fibers coated with collagen: in vivo evaluation of the effects on bone repair

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dc.contributor.authorUeno, Fabio Roberto [UNIFESP]
dc.contributor.authorKido, Hueliton Wilian [UNIFESP]
dc.contributor.authorGranito, Renata Neves [UNIFESP]
dc.contributor.authorGabbai-Armelin, Paulo Roberto [UNIFESP]
dc.contributor.authorMagri, Angela Maria Paiva [UNIFESP]
dc.contributor.authorFernandes, Kelly Rossetti [UNIFESP]
dc.contributor.authorSilva, Antonio Carlos da
dc.contributor.authorBraga, Francisco José Correa
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.date.accessioned2019-01-21T10:29:48Z
dc.date.available2019-01-21T10:29:48Z
dc.date.issued2016
dc.description.abstractThe aim of this study was to assess the characteristics of the CaP/Col composites, in powder and fiber form, via scanning electron microscopy (SEM), pH and calcium release evaluation after immersion in SBF and to evaluate the performance of these materials on the bone repair process in a tibial bone defect model. For this, four different formulations (CaP powder -CaPp, CaP powder with collagen -CaPp/Col, CaP fibers - CaPf and CaP fibers with collagen - CaPf/Col) were developed. SEM images indicated that both material forms were successfully coated with collagen and that CaPp and CaPf presented HCA precursor crystals on their surface. Although presenting different forms, FTIR analysis indicated that CaPp and CaPf maintained the characteristic peaks for this class of material. Additionally, the calcium assay study demonstrated a higher Ca uptake for CaPp compared to CaPf for up to 5 days. Furthermore, pH measurements revealed that the collagen coating prevented the acidification of the medium, leading to higher pH values for CaPp/Col and CaPf/Col. The histological analysis showed that CaPf/Col demonstrated a higher amount of newly formed bone in the region of the defect and a reduced presence of material. In summary, the results indicated that the fibrous CaP enriched with the organic part (collagen) glassy scaffold presented good degradability and bone-forming properties and also supported Runx2 and RANKL expression. These results show that the present CaP/Col fibrous composite may be used as a bone graft for inducing bone repair.en
dc.description.affiliationDepartment of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil
dc.description.affiliationNuclear and Energy Research Institute (IPEN), São Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Biosciences, Federal University of São Paulo (UNIFESP), Rua Silva Jardim, 136, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent259-273
dc.identifierhttps://dx.doi.org/10.3233/BME-161581
dc.identifier.citationBio-Medical Materials And Engineering. Amsterdam, v. 27, n. 43161, p. 259-273, 2016.
dc.identifier.doi10.3233/BME-161581
dc.identifier.issn0959-2989
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/49406
dc.identifier.wosWOS:000383187600012
dc.language.isoeng
dc.publisherCadernos Saude Publica
dc.relation.ispartofBio-Medical Materials And Engineering
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCompositeen
dc.subjectCalcium Phosphateen
dc.subjectCollagenen
dc.subjectFibersen
dc.subjectBone RepairBioactive Glassen
dc.subjectVitro Degradationen
dc.subjectCompositesen
dc.subjectOsteoblasten
dc.subjectScaffolden
dc.subjectCementen
dc.subjectRegenerationen
dc.subjectBiocompatibilityen
dc.subjectHydroxyapatiteen
dc.subjectMineralizationen
dc.titleCalcium phosphate fibers coated with collagen: in vivo evaluation of the effects on bone repairen
dc.typeinfo:eu-repo/semantics/article
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