Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein

dc.contributor.authorPereira, Catia Mendes [UNIFESP]
dc.contributor.authorFranco da Silveira, José [UNIFESP]
dc.contributor.authorDuranti, M.
dc.contributor.authorCunha-Neto, E.
dc.contributor.authorZingales, B.
dc.contributor.authorCastilho, Beatriz Amaral de [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:37:12Z
dc.date.available2016-01-24T12:37:12Z
dc.date.issued2004-06-15
dc.description.abstractB13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.en
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-01023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Inst Coracao, Lab Immunol Transplantes, BR-01498 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-01023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent237-242
dc.identifierhttp://dx.doi.org/10.1016/j.femsle.2004.04.040
dc.identifier.citationFems Microbiology Letters. Amsterdam: Elsevier B.V., v. 235, n. 2, p. 237-242, 2004.
dc.identifier.doi10.1016/j.femsle.2004.04.040
dc.identifier.issn0378-1097
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27792
dc.identifier.wosWOS:000222144400004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFems Microbiology Letters
dc.rightsAcesso aberto
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjectimmunodominant repetitive antigenen
dc.subjectB13 antigenen
dc.subjectB cell epitope mappingen
dc.subjectEscherichia coli LamB proteinen
dc.titleEpitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB proteinen
dc.typeArtigo
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