Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
dc.contributor.author | Pereira, Catia Mendes [UNIFESP] | |
dc.contributor.author | Franco da Silveira, José [UNIFESP] | |
dc.contributor.author | Duranti, M. | |
dc.contributor.author | Cunha-Neto, E. | |
dc.contributor.author | Zingales, B. | |
dc.contributor.author | Castilho, Beatriz Amaral de [UNIFESP] | |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2016-01-24T12:37:12Z | |
dc.date.available | 2016-01-24T12:37:12Z | |
dc.date.issued | 2004-06-15 | |
dc.description.abstract | B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies. | en |
dc.description.affiliation | UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-01023062 São Paulo, Brazil | |
dc.description.affiliation | Univ São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, Brazil | |
dc.description.affiliation | Univ São Paulo, Fac Med, Inst Coracao, Lab Immunol Transplantes, BR-01498 São Paulo, Brazil | |
dc.description.affiliationUnifesp | UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-01023062 São Paulo, Brazil | |
dc.description.source | Web of Science | |
dc.format.extent | 237-242 | |
dc.identifier | http://dx.doi.org/10.1016/j.femsle.2004.04.040 | |
dc.identifier.citation | Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 235, n. 2, p. 237-242, 2004. | |
dc.identifier.doi | 10.1016/j.femsle.2004.04.040 | |
dc.identifier.issn | 0378-1097 | |
dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/27792 | |
dc.identifier.wos | WOS:000222144400004 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Fems Microbiology Letters | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.subject | Trypanosoma cruzi | en |
dc.subject | immunodominant repetitive antigen | en |
dc.subject | B13 antigen | en |
dc.subject | B cell epitope mapping | en |
dc.subject | Escherichia coli LamB protein | en |
dc.title | Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein | en |
dc.type | info:eu-repo/semantics/article |