DNA and bone structure preservation in medieval human skeletons

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dc.contributor.authorCoulson-Thomas, Yvette May [UNIFESP]
dc.contributor.authorNorton, Andrew L.
dc.contributor.authorCoulson-Thomas, Vivien Jane [UNIFESP]
dc.contributor.authorFlorencio-Silva, Rinaldo [UNIFESP]
dc.contributor.authorAli, Nadir
dc.contributor.authorElmrghni, Samir
dc.contributor.authorGil, Cristiane D. [UNIFESP]
dc.contributor.authorSasso, Gisela R. S. [UNIFESP]
dc.contributor.authorDixon, Ronald A.
dc.contributor.authorNader, Helena B. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionLincoln Univ
dc.contributor.institutionUniv Durham
dc.contributor.institutionUniv Cambridge
dc.date.accessioned2016-01-24T14:40:33Z
dc.date.available2016-01-24T14:40:33Z
dc.date.issued2015-06-01
dc.description.abstractMorphological and ultrastructural data from archaeological human bones are scarce, particularly data that have been correlated with information on the preservation of molecules such as DNA. Here we examine the bone structure of macroscopically well-preserved medieval human skeletons by transmission electron microscopy and immunohistochemistry, and the quantity and quality of DNA extracted from these skeletons. DNA technology has been increasingly used for analyzing physical evidence in archaeological forensics; however, the isolation of ancient DNA is difficult since it is highly degraded, extraction yields are low and the co-extraction of PCR inhibitors is a problem. We adapted and optimised a method that is frequently used for isolating DNA from modern samples, Chelex (R) 100 (BioRad) extraction, for isolating DNA from archaeological human bones and teeth. the isolated DNA was analysed by real-time PCR using primers targeting the sex determining region on the Y chromosome (SRY) and STR typing using the AmpFlSTR (R) Identifiler PCR Amplification kit. Our results clearly show the preservation of bone matrix in medieval bones and the presence of intact osteocytes with well preserved encapsulated nuclei. in addition, we show how effective Chelex1 100 is for isolating ancient DNA from archaeological bones and teeth. This optimised method is suitable for STR typing using kits aimed specifically at degraded and difficult DNA templates since amplicons of up to 250 bp were successfully amplified. (C) 2015 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationLincoln Univ, Sch Life Sci, Lincoln LN6 7TS, England
dc.description.affiliationUniv Durham, Durham DH7 9RH, England
dc.description.affiliationUniv Cambridge, John van Geest Ctr Brain Repair, Cambridge CB2 0PY, England
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent186-194
dc.identifierhttp://dx.doi.org/10.1016/j.forsciint.2015.04.005
dc.identifier.citationForensic Science International. Clare: Elsevier B.V., v. 251, p. 186-194, 2015.
dc.identifier.doi10.1016/j.forsciint.2015.04.005
dc.identifier.issn0379-0738
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/39126
dc.identifier.wosWOS:000354125900028
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofForensic Science International
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAncient DNAen
dc.subjectArchaeological boneen
dc.subjectArchaeological teethen
dc.subjectChelexen
dc.subjectDNA typingen
dc.subjectElectron microscopyen
dc.titleDNA and bone structure preservation in medieval human skeletonsen
dc.typeArtigo
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