Thermoprotective effect of insulin-like growth factor 1 on in vitro matured bovine oocyte exposed to heat shock

Página do item simplificado
dc.citation.issue8
dc.citation.volume86
dc.contributor.authorRodrigues, Thais Alves [UNIFESP]
dc.contributor.authorIspada, Jessica [UNIFESP]
dc.contributor.authorRisolia, Pedro Henrique Bugallo
dc.contributor.authorRodrigues, Mariana Teixeira [UNIFESP]
dc.contributor.authorLima, Rafaela S.
dc.contributor.authorAssumpcao, Mayra Elena Ortiz D'Avila
dc.contributor.authorVisintin, José Antônio
dc.contributor.authorPaula-Lopes, Fabiola Freitas [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-07-31T12:47:31Z
dc.date.available2020-07-31T12:47:31Z
dc.date.issued2016
dc.description.abstractThe role of insulin-like growth factor 1 (IGF1) on cellular function and developmental capacity of heat-shocked oocytes has not been completely understood. Therefore, the objective of this study was to determine the effect of IGF1 on apoptosis, mitochondrial activity, cytoskeletal changes, nuclear maturation, and developmental competence of bovine oocytes exposed to heat shock. Cumulus-oocyte complexes were submitted to control (38.5 degrees C for 22 hours) and heat shock (41 degrees C for 14 hours followed by 38.5 degrees C for 8 hours) in the presence of 0 or 100 ng/mL IGF1 during IVM. Heat shock increased the percentage of TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling)-positive oocyte and reduced oocyte mitochondrial activity. However, addition of 100 ng/mL IGF1 minimized these deleterious effects of temperature. Caspase activity was affected neither by heat shock nor IGF1. Exposure of bovine oocytes to 41 degrees C during the first 14-hour IVM affected cortical actin localization and microtubule organization at the meiotic spindle and reduced the percentage oocytes that reached the metaphase II stage. However, in the presence of IGF1, cortical actin and percentage of metaphase II oocytes were not different between control and heat-shocked oocytes, suggesting a partial beneficial effect of IGF1. There was no effect of IGF1 on microtubule organization. Heat shock also reduced the percentage of oocytes that reached the blastocyst stage, blastocyst cell number, and increased the percentage of TUNEL-positive blastomeres. However, there was no effect of 100 ng/mL IGF1 on oocyte development to the blastocyst stage and blastocyst quality. Therefore, 100 ng/mL IGF1 prevented some heat shock-induced cellular damage in bovine oocytes but had no effect on oocyte developmental competence. In contrast, a low IGF1 concentration (25 ng/mL) had a thermoprotective effect on oocyte developmental competence to the blastocyst stage. In conclusion, IGF1 prevented part of the damage induced by heat shock on oocyte function. This effect was modulated by IGF1 concentration. (C) 2016 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biol Sci, Diadema, SP, Brazil
dc.description.affiliationState Univ Sao Paulo, Inst Biosci, Botucatu, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Anim Reprod, Sao Paulo, Brazil
dc.description.affiliationUnifespDepartment of Biological Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2007/53323-0
dc.description.sponsorshipIDCNPq: 478558/2008-2
dc.description.sponsorshipIDCNPq: 501205/2009-8
dc.format.extent2028-2039
dc.identifierhttps://dx.doi.org/10.1016/j.theriogenology.2016.06.023
dc.identifier.citationTheriogenology. New York, v. 86, n. 8, p. 2028-2039, 2016.
dc.identifier.doi10.1016/j.theriogenology.2016.06.023
dc.identifier.issn0093-691X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56878
dc.identifier.wosWOS:000384628200022
dc.language.isoeng
dc.publisherElsevier Science Inc
dc.relation.ispartofTheriogenology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectIGF1en
dc.subjectMaturationen
dc.subjectApoptosisen
dc.subjectMitochondrial activityen
dc.subjectCytoskeletonen
dc.subjectEmbryo developmenten
dc.titleThermoprotective effect of insulin-like growth factor 1 on in vitro matured bovine oocyte exposed to heat shocken
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICAQF - Artigos