Detecting Mechanisms of Karyotype Evolution in Heterotaxis (Orchidaceae)

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dc.citation.issue11
dc.citation.volume11
dc.contributor.authorMoraes, Ana Paula [UNIFESP]
dc.contributor.authorSimoes, Andre Olmos
dc.contributor.authorAlayon, Dario Isidro Ojeda
dc.contributor.authorde Barros, Fabio
dc.contributor.authorForni-Martins, Eliana Regina
dc.coverageSan Francisco
dc.date.accessioned2020-07-31T12:47:17Z
dc.date.available2020-07-31T12:47:17Z
dc.date.issued2016
dc.description.abstractThe karyotype is shaped by different chromosome rearrangements during species evolution. However, determining which rearrangements are responsible for karyotype changes is a challenging task and the combination of a robust phylogeny with refined karyotype characterization, GS measurements and bioinformatic modelling is necessary. Here, this approach was applied in Heterotaxis to determine what chromosome rearrangements were responsible for the dysploidy variation. We used two datasets (nrDNA and cpDNA, both under MP and BI) to infer the phylogenetic relationships among Heterotaxis species and the closely related genera Nitidobulbon and Ornithidium. Such phylogenies were used as framework to infer how karyotype evolution occurred using statistical methods. The nrDNA recovered Ornithidium, Nitidobulbon and Heterotaxis as monophyletic under both MP and BIen
dc.description.abstractwhile cpDNA could not completely separate the three genera under both methods. Based on the GS, we recovered two groups within Heterotaxis: (1) "small GS", corresponding to the Sessilis grade, composed of plants with smaller genomes and smaller morphological structure, and (2) "large GS", corresponding to the Discolor Glade, composed of plants with large genomes and robust morphological structures. The robust karyotype modeling, using both nrDNA phylogenies, allowed us to infer that the ancestral Heterotaxis karyotype presented 2n = 40, probably with a proximal 45S rDNA on a metacentric chromosome pair. The chromosome number variation was caused by ascending dysploidy (chromosome fission involving the proximal 45S rDNA site resulting in two acrocentric chromosome pairs holding a terminal 45S rDNA), with subsequent descending dysploidy (fusion) in two species, H. maleolens and H. sessilis. However, besides dysploidy, our analysis detected another important chromosome rearrangement in the Orchidaceae: chromosome inversion, that promoted 5S rDNA site duplication and relocation.en
dc.description.affiliationUniv Estadual Campinas UNICAMP, Inst Biol, Dept Biol Vegetal, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Inst Biociencias, Dept Genet, Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Inst Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv British Columbia, Biodivers Res Ctr, Vancouver, BC, Canada
dc.description.affiliationUniv British Columbia, Dept Bot, Vancouver, BC, Canada
dc.description.affiliationInst Bot, Sao Paulo, Brazil
dc.description.affiliationUnifespInstituto de Ciência e Tecnologia, Universidade Federal de São Paulo/UNIFESP, São José dos Campos, São Paulo, Brasil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCONACyT
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2008/03673-8
dc.description.sponsorshipIDFAPESP: 2011/22215-3
dc.description.sponsorshipIDCNPq: 304506/2013-3
dc.description.sponsorshipIDCNPq: 06142/2011-2
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0165960
dc.identifier.citationPlos One. San Francisco, v. 11, n. 11, p. -, 2016.
dc.identifier.doi10.1371/journal.pone.0165960
dc.identifier.fileWOS000387725000047.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56720
dc.identifier.wosWOS:000387725000047
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleDetecting Mechanisms of Karyotype Evolution in Heterotaxis (Orchidaceae)en
dc.typeinfo:eu-repo/semantics/article
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