Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil

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dc.citation.issue6]
dc.citation.volume12]
dc.contributor.authorVieira Cunha, Marcos Paulo
dc.contributor.authorSaidenberg, Andre Becker
dc.contributor.authorMoreno, Andrea Micke
dc.contributor.authorPiantino Ferreira, Antonio Jose
dc.contributor.authorMidolli Vieira, Monica Aparecida[UNIFESP]
dc.contributor.authorTardelli Gomes, Tania Aparecida [UNIFESP]
dc.contributor.authorKnobl, Terezinha
dc.coverageSan Francisco
dc.date.accessioned2020-06-26T16:30:37Z
dc.date.available2020-06-26T16:30:37Z
dc.date.issued2017
dc.description.abstractExtra-intestinal pathogenic Escherichia coli (ExPEC) represent an emerging pathogen, with pandemic strains increasingly involved in cases of urinary tract infections (UTIs), bacteremia, and meningitis. In addition to affecting humans, the avian pathotype of ExPEC, avian pathogenic E. coli (APEC), causes severe economic losses to the poultry industry. Several studies have revealed overlapping characteristics between APEC and human ExPEC, leading to the hypothesis of a zoonotic potential of poultry strains. However, the description of certain important pandemic clones, such as Sequence Type 73 (ST73), has not been reported in food sources. We characterized 27 temporally matched APEC strains from diverse poultry farms in Brazil belonging to the O6 serogroup because this serogroup is frequently described as a causal factor in UTI and septicemia in humans in Brazil and worldwide. The isolates were genotypically characterized by identifying ExPEC virulence factors, phylogenetically tested by phylogrouping and multilocus sequence type (MLST) analysis, and compared to determine their similarity employing the pulsed field gel electrophoresis (PFGE) technique. The strains harbored a large number of virulence determinants that are commonly described in uropathogenic E. coli (UPEC) and sepsis associated E. coli (SEPEC) strains and, to a lesser extent in neonatal meningitis associated E. coli (NMEC), such as pap (85%), sfa (100%), usp (100%), cnf1 (22%), kpsMTII (66%), hlyA (52%), and ibeA (4%). These isolates also yielded a low prevalence of some genes that are frequently described in APEC, such as iss (37%), tsh, ompT, and hlyF (8% each), and cvi/cva (0%). All strains were classified as part of the B2 phylogroup and sequence type 73 (ST73), with a cluster of 25 strains showing a clonal profile by PFGE. These results further suggest the zoonotic potential of some APEC clonal lineages and their possible role in the epidemiology of human ExPEC, in addition to providing the first description of the O6-B2-ST73 clonal group in poultry.en
dc.description.affiliationUniv Sao Paulo, Dept Pathol, Fac Med Vet & Zootecnia, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Vet Prevent Med, Fac Med Vet & Zootecnia, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP
dc.description.sponsorshipIDFAPESP: 05/57500-9-T
dc.description.sponsorshipIDFAPESP: 11/182046-T
dc.description.sponsorshipIDFAPESP: 14/11523-7]
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0178970]
dc.identifier.citationPlos One. San Francisco, v. 12, n. 6, p. -, 2017.
dc.identifier.doi10.1371/journal.pone.0178970
dc.identifier.fileWOS000402923200072.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53665
dc.identifier.wosWOS:000402923200072
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titlePandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazilen
dc.typeinfo:eu-repo/semantics/article
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