Primary sequence determination of a Kunitz inhibitor isolated from Delonix regia seeds

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dc.contributor.authorPando, S. C.
dc.contributor.authorOliva, MLV
dc.contributor.authorSampaio, CAM
dc.contributor.authorDi Ciero, L.
dc.contributor.authorNovello, J. C.
dc.contributor.authorMarangoni, S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2016-01-24T12:31:26Z
dc.date.available2016-01-24T12:31:26Z
dc.date.issued2001-07-01
dc.description.abstractA serine proteinase inhibitor was purified from Delonix regia seeds a Leguminosae tree of the Caesalpinioideae subfamily. the inhibitor named DrTI, inactivated trypsin and human plasma kallikrein with K-i values 2.19x10(-8) M and 5.25 nM, respectively. Its analysis by SDS-PAGE 10-20% showed that the inhibitor is a protein with a single polypeptide chain of M-r 22 h Da. the primary sequence of the inhibitor was determined by Edman degradation, thus indicating that it contained 185 amino acids and showed that it belongs to the Kunitz type family; however, its reactive site did not contain Arg or Lys at the putative reactive site (position 63, SbTI numbering) or it was displaced when compared to other Kunitz-type inhibitors. (C) 2001 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Biochem, BR-04044900 São Paulo, Brazil
dc.description.affiliationUNICAMP, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Biochem, BR-04044900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent625-631
dc.identifierhttp://dx.doi.org/10.1016/S0031-9422(01)00080-2
dc.identifier.citationPhytochemistry. Oxford: Pergamon-Elsevier B.V., v. 57, n. 5, p. 625-631, 2001.
dc.identifier.doi10.1016/S0031-9422(01)00080-2
dc.identifier.issn0031-9422
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26589
dc.identifier.wosWOS:000169481100002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPhytochemistry
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectDelonix regiaen
dc.subjectLeguminosaeen
dc.subjectKunitz inhibitoren
dc.subjectserine proteinaseen
dc.subjecttrypsinen
dc.subjecthuman plasma kallikreinen
dc.titlePrimary sequence determination of a Kunitz inhibitor isolated from Delonix regia seedsen
dc.typeinfo:eu-repo/semantics/article
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