Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

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dc.citation.volume98
dc.contributor.authorFlorencio-Silva, Rinaldo [UNIFESP]
dc.contributor.authorSasso, Gisela R. S.[UNIFESP]
dc.contributor.authorSasso-Cerri, Estela[UNIFESP]
dc.contributor.authorSimoes, Manuel J.
dc.contributor.authorCerri, Paulo S.
dc.coverageIssy-Les-Moulineaux
dc.date.accessioned2020-07-08T13:09:45Z
dc.date.available2020-07-08T13:09:45Z
dc.date.issued2018
dc.description.abstractEstrogen maintains osteocyte viability, whereas its deficiency induces osteocyte apoptosis. As autophagy is important for osteocyte viability, we hypothesized whether the anti-apoptotic effect of estrogen is related to autophagy in osteocytes. Thirty adult female rats were sham-operated (SHAM) or ovariectomized (OVX). After three weeks, twelve rats of SHAM and OVX groups were killed before treatment (basal period), whereas the remaining rats received estrogen (OVXE) or vehicle (OVX) for 45 days. Fragments of maxilla containing alveolar process of the first molars were embedded in paraffin or Araldite. Paraffin-sections were stained with hematoxylin/eosin for histomorphometry, or subjected to the silver impregnation method for morphological analysis of osteocyte cytoplasmic processes. Autophagy was analyzed by immunohistochemical detections of beclin-1, MAP-LC3 alpha and p62, whereas apoptosis was evaluated by immunohistochemical detections of cleaved caspase-3 and BAX, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method and by ultrastructural analysis. Araldite-semithin sections were subjected to the Sudan-black method for detection of lipids. OVX-basal group showed high frequency of caspase-3-, TUNEL- and p62-positive osteocytes accompanied with low frequency of beclin-1- and MAP-LC3 alpha-positive osteocytes. At 45 days, OVXE group exhibited higher number of osteocytes, higher frequency of beclin-1- and MAP-LC3 alpha-positive osteocytes, and lower frequency of caspase-3, BAX-, TUNEL- and p62-positive osteocytes than OVX group. Significant reduction in bone area was observed in the OVX compared to OVXE and SHAM groups. The highest frequency of Sudan-Black-positive osteocytes and osteocytes with scarce cytoplasmic processes, or showing apoptotic features were mainly observed in OVX groups. Our results indicate that estrogen deficiency decreases autophagy and increases apoptosis, whereas estrogen replacement enhances osteocyte viability by inhibiting apoptosis and maintaining autophagy in alveolar process osteocytes. These results suggest that the anti-apoptotic effect of estrogen may be, at least in part, related to autophagy regulation in osteocytes.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Morfol & Genet, Disciplina Histol & Biol Estrutural, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Ginecol, Sao Paulo, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Sch Dent, Araraquara Lab Histol & Embryol, Araraquara, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Morfol & Genet, Disciplina Histol & Biol Estrutural, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Ginecol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq), Brazil
dc.description.sponsorshipCoordination of Improvement of Higher Level Personnel (CAPES), Brazil
dc.description.sponsorshipIDFAPESP: 2012/19428-8, 2012/22666-8
dc.format.extent406-415
dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2017.12.089
dc.identifier.citationBiomedicine & Pharmacotherapy. Issy-Les-Moulineaux, v. 98, p. 406-415, 2018.
dc.identifier.doi10.1016/j.biopha.2017.12.089
dc.identifier.issn0753-3322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54176
dc.identifier.wosWOS:000426104000051
dc.language.isoeng
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier
dc.relation.ispartofBiomedicine & Pharmacotherapy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOsteocytesen
dc.subjectAutophagyen
dc.subjectApoptosisen
dc.subjectEstrogenen
dc.subjectAlveolar processen
dc.titleEffects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized ratsen
dc.typeinfo:eu-repo/semantics/article
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