A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells

Página do item simplificado
dc.citation.volume7
dc.contributor.authorMoraes, Daniela A.
dc.contributor.authorSibov, Tatiana T. [UNIFESP]
dc.contributor.authorPavon, Lorena F. [UNIFESP]
dc.contributor.authorAlvim, Paula Q.
dc.contributor.authorBonadio, Raphael S.
dc.contributor.authorDa Silva, Jaqueline R.
dc.contributor.authorPic-Taylor, Aline
dc.contributor.authorToledo, Orlando A.
dc.contributor.authorMarti, Luciana C.
dc.contributor.authorAzevedo, Ricardo B.
dc.contributor.authorOliveira, Daniela M.
dc.coverageLondon
dc.date.accessioned2020-08-14T13:44:08Z
dc.date.available2020-08-14T13:44:08Z
dc.date.issued2016
dc.description.abstractBackground: Mesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs. Methods: We investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors. Results: The present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression. Conclusion: Our study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro.en
dc.description.affiliationUniv Brasilia, Dept Genrt & Morfol, Brasilia, DF, Brazil
dc.description.affiliationUniv Brasilia, Dept Ciencias Saude, Brasilia, DF, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Neurol & Neurocirurgia, Sao Paulo, SP, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, Inst Ensino & Pesquisa, Centro Pesquisa Expt Sao Paulo, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Brasilia UNB, IB Dept Genet & Morfol, Campus Univ Darcy Ribeiro, BR-70910970 Brasilia, DF, Brazil
dc.description.affiliationCtr Univ Distrito Fed UDF, Brasilia, DF, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Neurol & Neurocirurgia, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Pesquisa (Brazil)
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s13287-016-0359-3
dc.identifier.citationStem Cell Research & Therapy. London, v. 7, p. -, 2016.
dc.identifier.doi10.1186/s13287-016-0359-3
dc.identifier.fileWOS000384587700001.pdf
dc.identifier.issn1757-6512
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57527
dc.identifier.wosWOS:000384587700001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofStem Cell Research & Therapy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMesenchymal stem cellsen
dc.subjectMesenchymal stromal cellsen
dc.subjectCD90en
dc.subjectThy-1en
dc.subjectFibroblasten
dc.subjectDifferentiationen
dc.titleA reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cellsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000384587700001.pdf
Tamanho:
2.68 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos