Optical properties and antimicrobial effects of silver nanoparticles synthesized by femtosecond laser photoreduction

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dc.contributor.authorCourrol, Daniella dos Santos
dc.contributor.authorLopes, Carla Regina Borges [UNIFESP]
dc.contributor.authorCordeiro, Thiago da Silva
dc.contributor.authorFranzolin, Marcia Regina
dc.contributor.authorVieira Junior, Nilson Dias
dc.contributor.authorSarnad, Ricardo Elgul
dc.contributor.authorCourrol, Lilia Coronato [UNIFESP]
dc.date.accessioned2018-07-26T12:18:28Z
dc.date.available2018-07-26T12:18:28Z
dc.date.issued2018
dc.description.abstractSilver nanoparticles exhibit a powerful antimicrobial action showing a pronounced potential to be widely used against drug resistance bacteria. The present work describes the optical properties and antimicrobial effect of silver nanoparticles produced by femtosecond laser photoreduction of AgNO3 in the presence of tryptophan water solution. The advantages of this method are the absence of hazardous chemical reducing agents in the solution, and the versatile dimensional control achieved. The synthesized silver nanoparticles were characterized by absorption and fluorescence spectroscopy and their antibacterial activity were determined by monitoring the cell viability of Escherichia coli. The effects of the silver nanoparticles concentration and laser parameters (exposure time and pulse energy), on the formation of the nanoparticles, and its influence on the bacteria growth inhibition were studied. The prepared silver nanoparticles exhibited suitable antimicrobial properties. The results demonstrated that the nanoparticles concentration plays an important role in their bactericidal efficacy. The increase in the laser energy caused an increase in E. coli growth inhibition. Irradiations with energies around 300 mu j for 60 min presented high antimicrobial activity due to the presence of kynurenine, sub product of tryptophan photolysis. The first-time formation mechanism of tryptophan silver nanoparticles in high optical intensities was also discussed. (C) 2018 Elsevier Ltd. All rights reserved.en
dc.description.affiliationInst Butantan, Lab Bacteriol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Fis, Lab Lasers & Opt Biomed Aplicada, Diadema, SP, Brazil
dc.description.affiliationIPEN CNEN SP, Ctr Lasers & Aplicacoes, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Fis, Lab Lasers & Opt Biomed Aplicada, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq)
dc.description.sponsorshipSao Paulo State Research Support Foundation
dc.description.sponsorshipPAP
dc.description.sponsorshipIDFAPESP: 2014/06960-9
dc.format.extent233-238
dc.identifierhttp://dx.doi.org/10.1016/j.optlastec.2018.01.044
dc.identifier.citationOptics And Laser Technology. Oxford, v. 103, p. 233-238, 2018.
dc.identifier.doi10.1016/j.optlastec.2018.01.044
dc.identifier.issn0030-3992
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/45972
dc.identifier.wosWOS:000427339000031
dc.language.isoeng
dc.publisherElsevier Sci Ltd
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectSilver nanoparticlesen
dc.subjectMicroorganismsen
dc.subjectLaseren
dc.subjectFemtoseconden
dc.subjectTryptophanen
dc.titleOptical properties and antimicrobial effects of silver nanoparticles synthesized by femtosecond laser photoreductionen
dc.typeinfo:eu-repo/semantics/article
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