Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

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dc.citation.issue12
dc.citation.volume33
dc.contributor.authorBraga, Daniela Paes Almeida Ferreira [UNIFESP]
dc.contributor.authorSetti, Amanda Souza
dc.contributor.authorLo Turco, Edson Guimaraes [UNIFESP]
dc.contributor.authorCordeiro, Fernanda Bertuccez [UNIFESP]
dc.contributor.authorCabral, Elaine Cristina
dc.contributor.authorCortezzi, Sylvia Sanches
dc.contributor.authorOno, Erika
dc.contributor.authorSavio Figueira, Rita Cassia
dc.contributor.authorEberlin, Marcos Nogueira
dc.contributor.authorBorges Júnior, Edson [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-07-31T12:47:10Z
dc.date.available2020-07-31T12:47:10Z
dc.date.issued2016
dc.description.abstractThe goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.en
dc.description.affiliationFertil Med Grp, Ave Brigadeiro Luis Antonio 4545, BR-01401002 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Cirurgia, Disciplina Urol Area Reproducao Humana, Rua Embau 231, BR-04039060 Sao Paulo, SP, Brazil
dc.description.affiliationInst Sapientiae, Ctr Estudos & Pesquisa Reproducao Humana Assistid, Rua VieiraMaciel 62, BR-04503040 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Campinas UNICAMP, Ctr Pluridisciplinar Pesquisas Quim Biol & Agrono, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Campinas UNICAMP, Inst Quim, Lab ThoMSon Espectrometria Massas, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnifespDisciplina de Urologia, Área de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo—UNIFESP, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2013/50052-7
dc.format.extent1571-1583
dc.identifierhttp://dx.doi.org/10.1007/s10815-016-0800-7
dc.identifier.citationJournal Of Assisted Reproduction And Genetics. New York, v. 33, n. 12, p. 1571-1583, 2016.
dc.identifier.doi10.1007/s10815-016-0800-7
dc.identifier.issn1058-0468
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56636
dc.identifier.wosWOS:000390025000006
dc.language.isoeng
dc.publisherSpringer/Plenum Publishers
dc.relation.ispartofJournal Of Assisted Reproduction And Genetics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCumulus cellen
dc.subjectBlastocysten
dc.subjectMass spectrometryen
dc.subjectPregnancyen
dc.subjectProteomicsen
dc.titleProtein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy successen
dc.typeinfo:eu-repo/semantics/article
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